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首页> 外文期刊>International journal of environmental analytical chemistry >Sensitive and renewable picloram immunosensor based on paramagnetic immobilisation
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Sensitive and renewable picloram immunosensor based on paramagnetic immobilisation

机译：基于顺磁固定的灵敏可再生吡咯烷免疫传感器

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摘要

Picloram (4-amino-3,5,6-trichloro-2-pyridincarboxylic acid) is one of the chlorinated pesticides. It is widely used for control of wood plants, wheat, barley and wide range of broadleaf weeds as a plant growth regulator. An immunosensor was developed for detection of picloram concentration in compost extracts and river water. The laccase-picloram was prepared. The magnetic core-shell (Fe_3O_4-SiO_2) nanoparticles were modified with anti-picloram-IgG and attached to the surface of carbon paste electrode (CPE) with the aid of paramagnetism. Following competitive immunoreaction with picloram and the picloram-laccase to form immunocomplex, electrochemical measurement was carried out. After immunoassay, the electrode was immersed in glycin-hydrochloric acid buffer or polished with diamond paper for regeneration. The linear range for picloram detection was 1 × 10~(-4)-10 μg mL~(-1) with the correlation coefficient of 0.9936, and the detection limit is 1 × 10~(-4) μg mL~(-1). The laccase labelled on the picloram for competitive immunoassay showed good activity, and the current response was strong and stable in electrochemical detection. The current reached 95% of the steady-state current within about 100 s. The proposed immunosensor exhibited good precision, sensitivity, selectivity, reusability, and storage stability.
机译：吡氯仑（4-氨基-3,5,6-三氯-2-吡啶羧酸）是一种氯化农药。作为植物生长调节剂，它被广泛用于控制木本植物，小麦，大麦和各种阔叶杂草。开发了一种免疫传感器，用于检测堆肥提取物和河水中的吡咯仑浓度。制备了漆酶-吡咯烷。将磁性核-壳（Fe_3O_4-SiO_2）纳米粒子用抗吡喃抗-IgG修饰，并借助顺磁性将其附着在碳糊电极（CPE）的表面上。在与吡咯兰和吡咯兰-漆酶竞争性免疫反应以形成免疫复合物之后，进行电化学测量。免疫测定后，将电极浸入甘氨酸-盐酸缓冲液中，或用金刚石纸抛光以再生。吡咯仑的线性范围为1×10〜（-4）-10μgmL〜（-1），相关系数为0.9936，检出限为1×10〜（-4）μgmL〜（-1） ）。在吡咯烷酮上标记的用于竞争性免疫分析的漆酶显示出良好的活性，并且在电化学检测中电流响应强且稳定。电流在约100 s内达到稳态电流的95％。所提出的免疫传感器表现出良好的精度，灵敏度，选择性，可重复使用性和储存稳定性。

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  • 作者

    Guang-Ming Zeng; Yi Zhang; Lin Tang; Li-Juan Chen; Ya Pang; Chong-Ling Feng; Guo-He Huang; Cheng-Gang Niu;

  • 作者单位

    College of Environmental Science and Engineering, Hunan University, Changsha 410082, China,Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China;

    College of Environmental Science and Engineering, Hunan University, Changsha 410082, China,Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China;

    College of Environmental Science and Engineering, Hunan University, Changsha 410082, China,Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China;

    College of Environmental Science and Engineering, Hunan University, Changsha 410082, China,Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China;

    College of Environmental Science and Engineering, Hunan University, Changsha 410082, China,Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China;

    College of Environmental Science and Engineering, Hunan University, Changsha 410082, China,Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China;

    College of Environmental Science and Engineering, Hunan University, Changsha 410082, China,Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China;

    College of Environmental Science and Engineering, Hunan University, Changsha 410082, China,Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    immunosensor; picloram; magnetic nanoparticles; laccase; regeneration;

    机译：免疫传感器吡咯仑磁性纳米粒子漆酶再生;

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