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首页> 外文期刊>International journal of environmental analytical chemistry >A new HPIC-UV method to quantify phenolic acids in food and environmental samples
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A new HPIC-UV method to quantify phenolic acids in food and environmental samples

机译：HPIC-UV定量食品和环境样品中酚酸的新方法

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摘要

A simple and rapid HPIC method has been developed for the separation and quantification of nine phenolic acids (PAs): gallic acid, syringic acid, vanillic acid, sinapic acid, ferulic acid, p-coumaric acid, protocatechuic acid, caffeic acid and ellagic acid. Separation was carried out on an Dionex Ion-Pac AS-11 (250 mm × 4 mm I.D.) column with a Dionex Ion-Pac AG-11 (50 mm × 4 mm I.D) guard column. Elution was performed using 1000 mM sodium hydroxide (NaOH) and 500 mM sodium acetate (NaOAc) in a multi step binary gradient at a flow rate of 1 mL min~(-1). Detection was performed using diode array detector set at 230, 250, 280, and 330 nm. After optimisation of various parameters, the separation of the nine phenolic acids was achieved within 23 minutes with a good resolution. Peak areas for each compound showed good linearity (R2 ＞ 0.999) in a relatively wide concentration range. Detection limits were in the range of 10-530 μg L~(-1) at a signal-to-noise ratio 3:1 and the amount of phenolic acids determined were in the range 0.20-10.0 ng when 20 μl of sample was injected. The developed procedure was successfully applied for the determination of these compounds in food (green tea, tomato juice and wine samples) and environmental samples (soil, surface water, organic fertiliser, organic waste) with minimal sample preparation. Beside good performances (separation, resolution, sensitivity and linearity), the new method is very fast and not expensive which makes it interesting for both scientific research and routine analysis of food and environmental samples.
机译：已开发出一种简单快速的HPIC方法来分离和定量九种酚酸（PAs）：没食子酸，丁香酸，香草酸，芥子酸，阿魏酸，对香豆酸，原儿茶酸，咖啡酸和鞣花酸。在Dionex Ion-Pac AS-11（250 mm×4 mm I.D.）色谱柱和Dionex Ion-Pac AG-11（50 mm×4 mm I.D）保护柱上进行分离。使用1000 mM氢氧化钠（NaOH）和500 mM乙酸钠（NaOAc）进行多步二元梯度洗脱，流速为1 mL min〜（-1）。使用设置为230、250、280和330 nm的二极管阵列检测器进行检测。优化各种参数后，在23分钟内以良好的分离度分离了9种酚酸。每种化合物的峰面积在相对较宽的浓度范围内均显示出良好的线性（R2＞ 0.999）。进样比为3：1时，检出限为10-530μgL〜（-1），进样20μl时测定的酚酸量为0.20-10.0 ng 。该开发的方法成功地用于食品（绿茶，番茄汁和葡萄酒样品）和环境样品（土壤，地表水，有机肥料，有机废物）中这些化合物的测定，只需最少的样品制备。除了良好的性能（分离，分离度，灵敏度和线性）外，新方法还非常快速且不昂贵，这使得它对科学研究以及对食品和环境样品的常规分析都非常有趣。

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  • 作者

    Sreedhar Mallipattu; Ghislain Sevenier; Yves Dudal;

  • 作者单位

    Institut National de la Recherche Agronomique, UMR Climat, Sol et Environment, Batiment Sol, Domaine Saint Paul-Site Agroparc-F84914, Avignon, Cedex-9, France;

    rnInstitut National de la Recherche Agronomique, UMR Climat, Sol et Environment, Batiment Sol, Domaine Saint Paul-Site Agroparc-F84914, Avignon, Cedex-9, France;

    rnInstitut National de la Recherche Agronomique, UMR Climat, Sol et Environment, Batiment Sol, Domaine Saint Paul-Site Agroparc-F84914, Avignon, Cedex-9, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    phenolic acids; ion chromatography; UV detection; environmental samples;

    机译：酚酸离子色谱紫外线检测;环境样品;

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