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Spectrophotometric verification of biodegradation of phenol in a flow dynamic biocers-based bioreactor system

机译:分光光度法验证基于流动动态生物的生物反应器系统中苯酚的生物降解

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A quick fingerprint spectrophotometric procedure based on the absorbance in the ultraviolet spectrum region was developed to verify biodegradation of phenol in flowing contaminated water. The procedure was employed to test the functional feasibility of a biocers-packed bioreactor to filter phenol on a bench-scale fluidic dynamic system, which should simulate a macroscopic microorganism-based remediation system. The biocers contained Rhodococcus sp. P1, a phenol degrading bacteria. An influent with initial phenol concentration of 500 mg L~(-1) was fed into the bioreactor at different flow rates, and the effluent was continuously monitored for residual phenol concentration by coupling the fluid dynamic system to a UV-vis spectrophotometer. Within 3 days, the effluent from bioreactor reached a minimum residual concentration of phenol of < 40 mg L~(-1). Therefore, the aim of the current paper is to report results of the procedure for on-line spectrophotometric detection of phenol, and the feasibility of a biocers-packed bioreactor for degradation of phenol.
机译:建立了一种基于紫外光谱区吸光度的快速指纹分光光度法,以验证流动的污水中苯酚的生物降解。该程序用于测试装有生物医生的生物反应器在台式流体动力学系统上过滤苯酚的功能可行性,该系统应模拟基于宏观微生物的修复系统。生物学家包含红球菌属。 P1,苯酚降解细菌。将初始苯酚浓度为500 mg L〜(-1)的进料以不同流速进料到生物反应器中,并通过将流体动力学系统与紫外可见分光光度计耦合,连续监测废水中残留的苯酚浓度。在3天之内,生物反应器的出水达到了苯酚的最小残留浓度<40 mg L〜(-1)。因此,本论文的目的是报告在线分光光度法检测苯酚的程序的结果,以及用生化试剂包装的生物反应器降解苯酚的可行性。

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