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首页> 外文期刊>The International Journal of Environmental Studies >Simultaneous measurements of mitochondrial trans-membrane electric potential and oxygen consumption as biosensors for ecotoxicological research
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Simultaneous measurements of mitochondrial trans-membrane electric potential and oxygen consumption as biosensors for ecotoxicological research

机译：同时测量线粒体跨膜电位和耗氧量作为生态毒理学研究的生物传感器

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摘要

Pesticide toxicity databases usually include data on pure chemicals (active ingredients). Technical pesticides formulations, however, are mixtures with adjuvants as applied to fields/crops. Two formulations of the same pesticide can differ in their environmental fate and their toxicity. This work presents the evaluation of the toxicity of the dinitroaniline herbicide, pendimethalin by simultaneous analysis of its respiration rate and generation of a membrane potential (Δψ) in rat liver mitochondria. Chromatography grade pendimethalin (8.2·10~(-5) - 5.47·10~(-4) M or 23 - 154 ppm) caused lower enhancement of the mitochondrial respiration and decrease of the Δ ψ than technical grade pendimethalin (Stomp), i.e. the mixture of pure ingredient and adjuvant(s). These effects are comparable to those of 2,4-dinitrophenol. Pure and technical grade pendimethalin acts as an uncou-pler of oxidative phosphorylation in mitochondria (enhances respiration and diminishes Δ ψ). These data conflict with the statement, presented in the US EPA manual Recognition and Management of Pesticides Poisonings, that pendimethalin does not act as an uncoupler of oxidative phosphorylation.
机译：农药毒性数据库通常包括纯化学品（活性成分）的数据。但是，工业农药制剂是与佐剂混合的混合物，适用于田地/农作物。同一农药的两种配方在环境命运和毒性方面可能有所不同。这项工作通过同时分析其呼吸速率和在大鼠肝线粒体中产生膜电位（Δψ），对二硝基苯胺除草剂戊二甲灵的毒性进行了评估。色谱级二甲戊乐灵（8.2·10〜（-5）-5.47·10〜（-4）M或23-154 ppm）引起的线粒体呼吸增强作用降低，Δψ降低，低于工业级二甲戊乐灵（Stomp），即纯成分和佐剂的混合物。这些效果与2,4-二硝基苯酚相当。纯净的和工业级的戊二甲戊灵在线粒体中起氧化磷酸化的抑制作用（增强呼吸作用并降低Δψ）。这些数据与美国EPA手册《农药中毒的确认和管理》中提出的声明矛盾，二甲戊乐灵不充当氧化磷酸化的解偶联剂。

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