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首页> 外文期刊>International journal of food science & technology >Antimicrobial activities of high molecular weight water-soluble chitosans against selected gram-negative and gram-positive foodborne pathogens
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Antimicrobial activities of high molecular weight water-soluble chitosans against selected gram-negative and gram-positive foodborne pathogens

机译：高分子量水溶性壳聚糖对某些革兰氏阴性和革兰氏阳性食源性病原体的抗菌活性

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摘要

Antimicrobial activities of high molecular weight water-soluble chitosans (HMWWS) against selected Gram-negative and Gram-positive foodborne pathogens (initial inoculation of ca. 6.5 Log CFU mL~(-1)) were evaluated. Chitosans with 789 kDa and/or 1017 kDa were dissolved in aspartic acid (AS) to obtain 1-4% w/v solutions. Among HMWWS, only 4% 789 kDa AS chitosan reduced E. coli counts by 2 Log CFU mL~(-1) from 7.33 at 0 h to 5.16 Log CFU mL~(-1) at 96 h, and they were not effective against S. Typhimurium. Depending on the concentrations, HMWWS completely inhibited V. cholerae, V. vulnificus and V. parahaemolyticus as well as B. cereus and L. monocytogenes after 48 h or 96 h of incubation. Compared with the control (no HMWWS), 2% or 3% 1017 kDa AS chitosans showed about 3 Log CFU mL~(-1) lower (4.72-4.86 vs. 7.71) for S. aureus at 96 h of incubation.
机译：评估了高分子量水溶性壳聚糖（HMWWS）对选定的革兰氏阴性和革兰氏阳性食源性病原菌的抗菌活性（最初接种约6.5 Log CFU mL〜（-1））。将具有789 kDa和/或1017 kDa的壳聚糖溶于天冬氨酸（AS）中，以获得1-4％w / v的溶液。在HMWWS中，只有4％789 kDa AS壳聚糖将大肠杆菌计数从0 h的7.33降低2 Log CFU mL〜（-1）降至96 h的5.16 Log CFU mL〜（-1），并且对鼠伤寒沙门氏菌。温育48小时或96小时后，根据浓度，HMWWS完全抑制霍乱弧菌，创伤弧菌和副溶血弧菌以及蜡状芽孢杆菌和单核细胞增生李斯特菌。与对照（无HMWWS）相比，在培养96小时后，金黄色葡萄球菌的2％或3％1017 kDa AS壳聚糖显示金黄色葡萄球菌的CFU mL〜（-1）降低了约3 Log CFU mL〜（-1）（4.72-4.86对7.71）。

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