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首页> 外文期刊>International Journal of Heat and Fluid Flow >Electrical classification of single red blood cell deformability in high-shear microchannel flows
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Electrical classification of single red blood cell deformability in high-shear microchannel flows

机译:高剪切微通道血流中单红细胞变形能力的电分类

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摘要

A sensor that can efficiently and sequentially measure the deformability of individual red blood cell (RBC) flowing along a microchannel is described. Counter-electrode-type microsensors are attached to the channel bottom wall, and as RBCs pass between the electrodes, the time series of the electric resistance is measured. An RBC is deformed by the high shear flow to a degree dependent upon its elastic modulus. Hence, the profile of the resistance, which is unique to the shape of the RBC, can be analyzed to obtain the deformability of each cell. First, theoretical and experimental analyses were conducted to identify the specific AC frequency at which the effect of the electric double layer formed on the electrode surface is minimized. Measurements were then conducted upon samples of normal human RBCs and glutaralde-hyde-treated (rigidified) RBCs to evaluate the feasibility of the present method. In addition, simultaneous visualization of RBC deformation was performed using a high-speed camera. Normal RBCs were observed to have a degree of deformation index (DI) of around 0.57, whereas the rigidified RBCs was DI-0 in the microchannel. The experimental measurements showed a strong correlation between the half-width of the maximum of the resistance distribution and the DI of the RBC.
机译:描述了一种传感器,该传感器可以有效且顺序地测量沿微通道流动的单个红细胞(RBC)的可变形性。反电极型微传感器安装在通道底壁上,当RBC在电极之间通过时,测量电阻的时间序列。 RBC在高剪切力作用下会变形,变形程度取决于其弹性模量。因此,可以分析对于RBC形状唯一的电阻分布,以获得每个单元的可变形性。首先,进行理论和实验分析,以确定特定的交流频率,在该频率下,在电极表面形成的双电层的影响最小。然后对正常人红细胞和戊二醛-处理过的(刚性的)红细胞样品进行测量,以评估本方法的可行性。此外,使用高速摄像机对RBC变形进行了同时可视化。观察到正常的RBC的变形指数(DI)约为0.57,而刚性RBC在微通道中的DI-0为DI-0。实验测量结果表明,最大电阻分布的半宽度与RBC的DI之间具有很强的相关性。

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