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首页> 外文期刊>International Journal of Heat and Mass Transfer >Thermal study of polymerase chain reaction with capillary tubes
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Thermal study of polymerase chain reaction with capillary tubes

机译：毛细管聚合酶链反应的热研究

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摘要

Polymerase Chain Reaction (PCR) amplifies specific deoxyribonucleic acid (DNA) targets whereby the reaction vessels are placed in high thermal inertia media and subjected to multiple cycles of discrete temperatures to effect denaturation, annealing and extension events. Precise temperature control and efficient heat transfer are crucial factors in achieving amplification reproducibility and reliability as well as in reducing the time required for PCR. Understanding the temperature distribution and transfer characteristics during PCR will facilitate system optimization to enhance the efficiency of DNA amplification. We describe a numerical thermal study investigating temperature profiles achieved using the classical method of manual transfer of PCR reaction vessels through a series of temperature-controlled media compared with that of a more recently reported method that displaces reaction vessels spatially by tilting through different temperature zones. It was found that while the classical method allows the reagent to achieve uniform temperature distribution, natural convection effects would necessitate translation speeds in excess of 150 mm/min in order to limit the reagent temperature drop to within 10 °C. With the tilt method, uniform temperature distributions as well as temperature settling times within 5 s could be attained. The simulation results demonstrated here indicate that the tilt method can be developed further to augment extreme PCR requiring less than 2s for each cycle.
机译：聚合酶链式反应（PCR）放大比脱氧核糖核酸（DNA）靶标，从而将反应容器置于高热惯性培养基中，并进行多个离散温度的循环，以实现变性，退火和延伸事件。精确的温度控制和有效的传热是实现扩增重现性和可靠性的关键因素，以及减少PCR所需的时间。理解PCR期间的温度分布和转移特性将促进系统优化以提高DNA扩增效率。我们描述了使用通过一系列温度控制的介质进行PCR反应容器的经典方法来实现的数值热研究，其使用PCR反应容器的经典方法与最近报道的方法相比，通过不同的温度区域倾斜通过不同的温度区域移动反应容器。结果发现，虽然经典方法允许试剂实现均匀的温度分布，但是自然对流效应将需要超过150mm / min的翻译速度，以将试剂温度降至10℃。利用倾斜法，可以获得均匀的温度分布以及5秒内的温度沉降时间。这里证明的模拟结果表明，倾斜方法可以进一步开发，以增加每个循环的极端PCR需要小于2S的极端PCR。

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  • 来源
    《International Journal of Heat and Mass Transfer》 |2021年第9期|121508.1-121508.8|共8页
  • 作者

    Hassan Ali Abid; Jian Wern Ong; Zhixiong Song; Eric Shen Lin; Oi Wah Liew; Tuck Wah Ng;

  • 作者单位

    Laboratory for Optics and Applied Mechanics Department of Mechanical & Aerospace Engineering Monash University Clayton Victoria 3800 Australia;

    Laboratory for Optics and Applied Mechanics Department of Mechanical & Aerospace Engineering Monash University Clayton Victoria 3800 Australia;

    Laboratory for Optics and Applied Mechanics Department of Mechanical & Aerospace Engineering Monash University Clayton Victoria 3800 Australia;

    Laboratory for Optics and Applied Mechanics Department of Mechanical & Aerospace Engineering Monash University Clayton Victoria 3800 Australia;

    Cardiovascular Research Institute Yong Loo Lin School of Medicine National University of Singapore. National University Health System Centre for Translational Medicine 14 Medical Drive 117599 Singapore;

    Laboratory for Optics and Applied Mechanics Department of Mechanical & Aerospace Engineering Monash University Clayton Victoria 3800 Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Polymerase chain reaction; Thermal cycling; DNA; Capillary tubes;

    机译：聚合酶链反应;热循环;脱氧核糖核酸;毛细管;

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