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首页> 外文期刊>International Journal of Heat and Mass Transfer >Intracellular ice formation (IIF) during freeze-thaw repetitions
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Intracellular ice formation (IIF) during freeze-thaw repetitions

机译：冻融重复过程中的细胞内冰形成（IIF）

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摘要

Intracellular ice formation (IIF) plays a crucial role in cryobiology. The cell membrane is believed to play important roles in IIF initiation, however the underlying biophysical mechanisms are still not entirely understood. In this work we use a unidirectional freezing design for observations of the freezing process across the cell membrane, which then allow cell membrane integrity detection via dual fluorescent staining. Important IIF information including the location of the initiation site, the growth rate, and the ice dendrite morphology was well obtained. It is found that IIF always initiates at the cell membrane in contact with extracellular ice. Cell membranes are found to be disrupted after IIF in the high freezing rate region (>20 ℃/min), however they are observed to keep their integrity after IIF in the low freezing rate region (<20 ℃/min). The extracellular to intracellular ice penetration seems to be easier and the ice den-drites become larger when IIF is applied again to the intact cells in subsequent freezing cycles. The results give insight into the IIF mechanisms as well as the relationship between IIF and the cell membrane.
机译：细胞内冰的形成（IIF）在冷冻生物学中起着至关重要的作用。人们认为细胞膜在IIF启动中起重要作用，但是其潜在的生物物理机制仍未完全了解。在这项工作中，我们使用单向冷冻设计来观察整个细胞膜的冷冻过程，然后通过双重荧光染色进行细胞膜完整性检测。很好地获得了重要的IIF信息，包括起始位点的位置，生长速率和冰枝晶形态。发现IIF总是在与细胞外冰接触的细胞膜上起始。在高冷冻速率区域（> 20℃/ min）中，IIF后发现细胞膜被破坏，但是在低冷冻速率区域（<20℃/ min）中，IIF后细胞膜保持完整。当在随后的冷冻循环中将IIF再次施加至完整细胞时，细胞外至细胞内的冰渗透似乎更容易，并且冰的树突变大。结果提供了对IIF机制以及IIF与细胞膜之间关系的深入了解。

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  • 来源
  • 作者

    Yuhui Li; Hao Wang; Tingrui Pan;

  • 作者单位

    Lab of Heat and Mass Transport at Micro-Nano Scale, College of Engineering, Peking University, Beijing 100871, China;

    Lab of Heat and Mass Transport at Micro-Nano Scale, College of Engineering, Peking University, Beijing 100871, China,Beijing Key Laboratory for Solid Waste Utilization and Management, College of Engineering, Peking University, Beijing 100871, China;

    Micro-Nano Innovations (MiNI) Laboratory, Biomedical Engineering, Davis 95616, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Intracellular ice formation (IIF); Freeze-thaw; Cell membrane; Unidirectional freezing;

    机译：细胞内冰形成（IIF）;冻融;细胞膜;单向冻结;

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