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Applications of a Confocal Scanning Laser Holography (CSLH) instrument for measuring the three-dimensional temperature of a fluid and transparent objects

机译:共聚焦扫描激光全息术(CSLH)仪器在测量流体和透明物体的三维温度中的应用

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The Confocal Scanning Laser Holography (CSLH) microscope was designed to measure the temperature distribution of a fluid in three dimensions using a focused laser beam. The laser beam passes through the specimen and is interfered with a reference beam to form a hologram. The minute changes in refractive index produce fringe-shifts in a hologram. The fringe-shifts are converted to temperature, pressure, or composition depending on the configuration. A tomographic reconstruction algorithm, which is based on the numerical aperture of the beam, was derived for the microscope. Narrow field angle scanning is restricted to the numerical aperture or cone angle of the laser beam probing the specimen which increases the error in determining the three-dimensional properties of a specimen. The holography aspect of the microscope preserves the phase of the object which provides a temperature sensitivity of 0.1 ℃ based on a 1/10 wave fringe shift resolution in the hologram. The reconstructed temperature resolution is 1 ℃ in three-dimensions by processing the experiment data. The CSLH concept and tomographic reconstruction method of hologram data can be applied to precise non-invasive measurement of displacement, temperature, pressure, and composition of thick regions with positional resolution near the wavelength of the laser beam. Micro-fluidics and other areas of research and applied technology may well consider the unique measurement benefits of the CSLH device.
机译:共聚焦扫描激光全息术(CSLH)显微镜设计为使用聚焦激光束在三个维度上测量流体的温度分布。激光束穿过样品并与参考光束发生干涉,形成全息图。折射率的微小变化会在全息图中产生条纹​​偏移。条纹位移会根据配置转换为温度,压力或成分。为显微镜得出了一种基于射线数值孔径的断层重建算法。窄视场角扫描仅限于探测样品的激光束的数值孔​​径或锥角,这增加了确定样品三维特性的误差。显微镜的全息图方面保留了物体的相位,基于全息图中1/10的波条纹偏移分辨率,该物体提供了0.1℃的温度灵敏度。通过处理实验数据,三维重建的温度分辨率为1℃。全息数据的CSLH概念和断层摄影重建方法可用于位移,温度,压力和厚区域组成的精确无创测量,其位置分辨率接近激光束的波长。微流体以及其他研究和应用技术领域可能会考虑CSLH设备的独特测量优势。

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