• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Heat and Mass Transfer >Mass diffusion coefficient measurements at the microscale: Imaging a transient concentration profile using TIRF microscopy
【24h】

Mass diffusion coefficient measurements at the microscale: Imaging a transient concentration profile using TIRF microscopy

机译：微观尺度的质量扩散系数测量：使用TIRF显微镜成像瞬态浓度曲线

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This article describes a new method to measure the mass diffusion coefficient of a fluorescent dye in a liquid, inside a millichannel geometry. The proposed method, based on Total Internal Reflection Fluorescence (TIRF) microscopy, enables the measurement of the diffusion coefficient for mass transport parallel to the millichannel wall. It determines the diffusion coefficient that provides the best fit of measured concentration profiles as a function of position and time, following a Heaviside-shaped initial concentration profile. A thorough description of the methodology is provided, including the mathematics of dye diffusion as well as fluorescence imaging considerations. In particular, we carefully develop the explicit relationship between acquired images by TIRF microscopy and actual dye concentration profiles inside the millichannel, which leads to the experimental determination of the dye diffusion coefficient. This is followed by the presentation of validation experiments with rhodamine 6G in deionized water. An average diffusion coefficient of 3.3 × 10~(-10) m~2/s is obtained, which is in good agreement with previously reported values.
机译：本文介绍了一种在毫通道几何形状内部测量荧光染料在液体中的质量扩散系数的新方法。所提出的方法基于全内反射荧光（TIRF）显微镜，能够测量平行于毫通道壁的质量传输的扩散系数。它根据Heaviside形状的初始浓度曲线确定扩散系数，该扩散系数提供最佳的实测浓度曲线作为位置和时间的函数。提供了对该方法的详尽描述，包括染料扩散的数学以及荧光成像方面的考虑。特别是，我们仔细开发了通过TIRF显微镜获得的图像与毫通道内部实际染料浓度曲线之间的显式关系，从而通过实验确定了染料扩散系数。接下来是罗丹明6G在去离子水中的验证实验。获得的平均扩散系数为3.3×10〜（-10）m〜2 / s，与先前报道的值非常吻合。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号