• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International journal of hydrogen energy >Characteristics of hydrogen and bioflocculant production by a transposon-mutagenized strain of Pantoea agglomerans BH18
【24h】

Characteristics of hydrogen and bioflocculant production by a transposon-mutagenized strain of Pantoea agglomerans BH18

机译：转座子突变体泛珠菌BH18的氢和生物絮凝剂生产特性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Based on the transposon-mutagenized library of Pantoea agglomerans BH18, the mutant strain 2-103 was obtained using mutant screens for high simultaneous production of hydrogen and bioflocculant. The mutant strain 2-103 was able to grow in medium with 50 mu g/mL of kanamycin and could be amplified using Tn7-based transposon PCR amplification in cells from several passages, indicating that the mutant strain 2-103 transposon insertion was stable. The mutant strain 2-103 cell culture from batch experiments with varying initial pH values, glucose concentrations and NaCl concentrations was determined. The result indicated that the optimum initial pH for hydrogen production and bioflocculant activity was 7.0 and 8.0, respectively. The optimal glucose concentration for hydrogen and bioflocculant production was 20 g/L. In addition, the mutant strain 2-103 could produce hydrogen and bioflocculant under marine and fresh culture conditions. The mutant strain 2-103 had the highest production of hydrogen and bioflocculant with (1.55 +/- 0.07) mol H-2/mol glucose and (83.7 +/- 5.89)% under marine culture conditions, which increased to (1.78 +/- 0.08) mol H-2/mol glucose and (93.5 +/- 6.24)% under fresh culture conditions. This study found that the mutant strain 2-103 has the ability to produce hydrogen and bioflocculant within a range of initial pH values, glucose and NaCl concentrations, which is beneficial for the improvement of cost-effective bio-treatment processes. (C) 2016 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.
机译：基于泛酸泛菌BH18的转座子诱变文库，使用突变筛选筛选出高产氢和生物絮凝剂的突变菌株2-103。突变株2-103能够在含有50μg / mL卡那霉素的培养基中生长，并且可以使用Tn7基转座子PCR扩增从细胞中传代数次，表明突变株2-103转座子的插入是稳定的。确定了来自批次实验的突变菌株2-103细胞培养物的初始pH值，葡萄糖浓度和NaCl浓度的变化。结果表明，产氢和生物絮凝剂活性的最佳初始pH分别为7.0和8.0。氢气和生物絮凝剂生产的最佳葡萄糖浓度为20 g / L。另外，突变菌株2-103在海洋和新鲜培养条件下可以产生氢和生物絮凝剂。突变菌株2-103的氢和生物絮凝剂的产量最高，在海洋养殖条件下为（1.55 +/- 0.07）mol H-2 / mol葡萄糖和（83.7 +/- 5.89）％，增加到（1.78 + / -在新鲜培养条件下为0.08）mol H-2 / mol葡萄糖和（93.5 +/- 6.24）％。这项研究发现，突变菌株2-103具有在一定的初始pH值，葡萄糖和NaCl浓度范围内产生氢和生物絮凝剂的能力，这有利于改进具有成本效益的生物处理工艺。 （C）2016氢能出版物有限公司。由Elsevier Ltd.出版。保留所有权利。

著录项

  • 来源
    《International journal of hydrogen energy》 |2016年第48期|22786-22792|共7页
  • 作者

    Liu Hongyan; Wang Hongyu; Qin Haihua;

  • 作者单位

    Tianjin Univ Sci & Technol, Tianjin Key Lab Marine Resources & Chem, Coll Marine & Environm Sci, Tianjin 300457, Peoples R China;

    Tianjin Univ Sci & Technol, Tianjin Key Lab Marine Resources & Chem, Coll Marine & Environm Sci, Tianjin 300457, Peoples R China;

    Tianjin Univ Sci & Technol, Tianjin Key Lab Marine Resources & Chem, Coll Marine & Environm Sci, Tianjin 300457, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Transposon-mutagenized library; Mutant screen; Hydrogen production; Bioflocculant activity;

    机译：转座子诱变文库;突变筛选;产氢;生物絮凝活性;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号