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首页> 外文期刊>International Journal of Peptide Research and Therapeutics >Variants of Lipopeptides Produced by Bacillus licheniformis HSN221 in Different Medium Components Evaluated by a Rapid Method ESI-MS
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Variants of Lipopeptides Produced by Bacillus licheniformis HSN221 in Different Medium Components Evaluated by a Rapid Method ESI-MS

机译：快速方法ESI-MS评估地衣芽孢杆菌HSN221在不同培养基成分中产生的脂肽变体

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摘要

A lipopeptide producing strain was isolated from an oil field and identified as Bacillus licheniformis HSN221. Nine different substrates were used to cultivate the strain under the same incubation conditions. Using a rapid method, Electrospray Ionization Mass Spectrometry (ESI-MS) combined with Thin Layer Chromatography (TLC), nine different lipopeptide homologues were found and identified. The strain produced four [Leu]surfactin homologues, surfactin C13, surfactin C14, surfactin C15 and surfactin C16, when cultivated in the medium with glucose, yeast extract and ammonium chloride, but it produced five lichenysin homologues, lichenysin C12, lichenysin C13, lichenysin C14, lichenysin C15 and lichenysin C16, when cultivated in the remaining eight media. Additionally, it showed that the type and relative content of each homologue were consistent with in each medium which is helpful for optimizing the medium components to cultivate the similar species.
机译：从油田分离出产生脂肽的菌株，并将其鉴定为地衣芽孢杆菌HSN221。在相同的孵育条件下，使用九种不同的底物来培养菌株。使用快速方法，电喷雾电离质谱（ESI-MS）结合薄层色谱（TLC），发现并鉴定了9种不同的脂肽同源物。当在含有葡萄糖，酵母提取物和氯化铵的培养基中培养时，该菌株产生四种表面活性素同系物，表面活性素C13，表面活性素C14，表面活性素C15和表面活性素C16，但是它产生了五个地衣霉素同源物，地衣霉素C12，地衣霉素C13，地衣霉素C14，地衣霉素C15和地衣霉素C16（在其余八种培养基中培养）。此外，它表明每种同源物的类型和相对含量与每种培养基中的一致，这有助于优化培养基成分以培养相似物种。

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  • 作者

    Yi-Ming Li; Namir I. A. Haddad; Shi-Zhong Yang; Bo-Zhong Mu;

  • 作者单位

    State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry East China University of Science and Technology Shanghai 200237 People’s Republic of China;

    State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry East China University of Science and Technology Shanghai 200237 People’s Republic of China;

    State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry East China University of Science and Technology Shanghai 200237 People’s Republic of China;

    State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry East China University of Science and Technology Shanghai 200237 People’s Republic of China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Lipopeptide; Bacillus licheniformis; Medium components; ESI-MS;

    机译：脂肽;地衣芽孢杆菌;中等成分;ESI-MS;

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