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首页> 外文期刊>International journal of plant sciences >THE RELATIONSHIP BETWEEN TAPETUM CELLS AND MICROSPORES BASED ON PROTEIN LOCALIZATION IN FRAXINUS ANGUSTIFOLIA (OLEACEAE) POLLEN GRAINS
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THE RELATIONSHIP BETWEEN TAPETUM CELLS AND MICROSPORES BASED ON PROTEIN LOCALIZATION IN FRAXINUS ANGUSTIFOLIA (OLEACEAE) POLLEN GRAINS

机译：基于蛋白质定位的金盏花花粉粒中脂多糖细胞与微孔的关系

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This study contributes to the knowledge of the traffic of substances between tapetum cells and microspores through the localization of pollen proteins during microsporogenesis, by using immunocytochemical techniques with TEM. Because the Oleaceae genera, including Olea and Fraxinus, share a very similar protein profile, an Ole e 1-like protein (which cross-reacts with the Olea europaea major allergen Ole e 1) was detected in Fraxinus angustifolia pollen grains. From the tetrad to mature-pollen grain stages, distinct labeling intensities were localized in the tapetal cells, anther locule, and orbicules as well as in the microspore and pollen cytoplasms and walls. The localization of this protein in the glycocalyx proves the special role of this layer in ectexine development. At exine deposition stages, the detection of immunogold particles in the anther locule and orbicules can be interpreted as showing an active transfer of these proteins from the tapetum cells to microspores. Moreover, at the bicellular stage, the diffusion of proteins from the pollen cytoplasm to the intine channels indicates their contribution to intine formation. These findings provide new evidence of the role of sporophytic and game-tophytic tissues in pollen grain development. Moreover, the Ole e 1-like protein may have an important role in modifying pollen grain walls.
机译：这项研究通过使用透射电镜的免疫细胞化学技术，通过在小孢子发生过程中通过花粉蛋白的定位来促进绒毡层细胞和小孢子之间物质运输的知识。因为包括油橄榄和水曲柳的豆科属具有非常相似的蛋白质谱，所以在水曲柳花粉粒中检测到了油样e-1样蛋白（与油橄榄欧洲主要变应原Ole e 1交叉反应）。从四倍体到成熟花粉阶段，不同的标记强度位于绒毛膜细胞，花药小室和小球以及小孢子和花粉细胞质和壁中。该蛋白在糖萼中的定位证明了该层在ectexine发育中的特殊作用。在外壁沉积阶段，在花药小室和小行星中检测到免疫金颗粒可解释为显示了这些蛋白质从绒毡层细胞到小孢子的主动转移。此外，在双细胞阶段，蛋白质从花粉细胞质向肌内通道的扩散表明它们对肌内形成的贡献。这些发现提供了孢子组织和配子体组织在花粉粒发育中的作用的新证据。此外，Ole e 1样蛋白可能在修饰花粉粒壁中起重要作用。

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