首页> 外文期刊>International journal of plant sciences >HYBRID ORIGIN OF PAEONIA × YANANENSIS REVEALED BY MICROSATELLITE MARKERS, CHLOROPLAST GENE SEQUENCES, AND MORPHOLOGICAL CHARACTERISTICS
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HYBRID ORIGIN OF PAEONIA × YANANENSIS REVEALED BY MICROSATELLITE MARKERS, CHLOROPLAST GENE SEQUENCES, AND MORPHOLOGICAL CHARACTERISTICS

机译:微卫星标记,叶绿体基因序列和形态学特征揭示的PA药×菜的杂交起源

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摘要

In Paeonia, hybridization is an important path for both the development of new cultivars and species formation in nature, but the characterization of hybrids has long been a problem. To establish the relationship among Paeonia yananensis, P. jishanensis, and P. rockii, we sampled 159 individuals from 11 populations around the core population of P. yananensis. Samples were subjected to morphological analysis of 22 characters and molecular analysis of three chloroplast DNA (cpDNA) fragments and 14 microsatellites. Paeonia yananensis was distinguishable from the other two species on the basis of morphological characteristics. The phylogenetic tree based on three intergenic spacers in the chloroplast genome (petB-petD, rpsl6-trnQ, and psbA-trnH) demonstrated that P. yananensis was very closely related to P. jishanensis. However, analysis of 14 microsatellite loci revealed that P. yananensis shared a considerable number of alleles with both P. jishanensis and P. rockii. These results demonstrated that P. yananensis (=P. × yananensis) is of hybrid origin, with P. jishanensis as a maternal parent and P. rockii as a paternal parent. Our success in documenting the hybrid origin of P. yananensis suggests that combined evidence from morphology, maternally inherited cpDNA, and biparentally inherited microsatellites could serve as a powerful tool in hybrid studies.
机译:在Pa药中,杂交是开发新品种和自然界中物种形成的重要途径,但杂交体的表征长期以来一直是一个问题。为了建立Pa药Pa药,鸡山rock药和假山P药之间的关系,我们从药的核心种群周围的11个种群中抽取了159个个体。对样品进行22个特征的形态分析,并对三个叶绿体DNA(cpDNA)片段和14个微卫星进行分子分析。根据形态特征,南e药可以与其他两个物种区分开。基于叶绿体基因组中的三个基因间隔区(petB-petD,rpsl6-trnQ和psbA-trnH)的系统树表明,延安疟原虫与吉山疟原虫密切相关。然而,对14个微卫星基因座的分析表明,延安山疟原虫与吉山山疟原虫和假山疟原虫共有大量等位基因。这些结果表明,延安假单胞菌(P. yananensis)是杂种起源,吉山山假单胞菌为母本,洛基假单胞菌为父本。我们成功地记录了P. yananensis的杂种起源,表明来自形态学,母本遗传的cpDNA和双亲遗传的微卫星的综合证据可以作为杂种研究的有力工具。

著录项

  • 来源
    《International journal of plant sciences》 |2010年第4期|P.409-420|共12页
  • 作者单位

    Landscape Architecture College of Beijing Forestry University, Beijing 100083, China Gansu Forestry Technological College, Tianshui, Gansu 741200, China;

    rnLandscape Architecture College of Beijing Forestry University, Beijing 100083, China National Flower Engineering Research Center, Key Laboratory for the Genetics and Breeding of Forest Trees and Ornamental Plants, Beijing 100083, China;

    rnState Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Xiangshan, Beijing 100093, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    chloroplast gene; hybrid origin; microsatellite loci; morphometrics; Paeonia x yananensis.;

    机译:叶绿体基因混合血统微卫星基因座形态计量学e药x延安。;
  • 入库时间 2022-08-18 03:45:40

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