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首页> 外文期刊>International journal of plant sciences >THE TEMPORAL AND SPATIAL EXPRESSION OF PR-5 LINUSITIN-LIKE GENE IN HEALTHY AND ETHYLENE-TREATED FLAX PLANTS
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THE TEMPORAL AND SPATIAL EXPRESSION OF PR-5 LINUSITIN-LIKE GENE IN HEALTHY AND ETHYLENE-TREATED FLAX PLANTS

机译：PR-5 linusintin样基因在健康和乙烯处理的亚麻植物中的时空表达

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摘要

Linusitin-like protein (LIN1) is a new osmotin-like protein isolated from flax radicles. The LIN1 gene is developmentally regulated and accumulated in an organ-specific manner in healthy flax plants. In an attempt to find the role and function of the LIN1 gene, we followed its temporal and spatial expression in different flax tissues during development and its induction after diverse osmotic stresses. The LIN1 mRNA was differentially expressed among the various organs during flax blooming. It was not detected in flowers, stems, or leaves, but a positive signal was found in roots. The major sites of LIN1 expression in roots were in the lateral roots. The LIN1 gene was activated in leaf by treatment with ethylene but not by methyl jasmonate or methyl salicylate. To understand its stress-response expression better, we isolated the promoter region of LIN1. Analysis of the promoter region revealed that it contains several ATATT "root motif" elements and one AGCCGCC sequence that is conserved in most basic pathogenesis-related (PR) protein genes and is responsible for ethylene induction.
机译：Linusitin-like蛋白（LIN1）是从亚麻胚根中分离出来的一种新的osmotin-like蛋白。 LIN1基因在健康的亚麻植物中以器官特异性方式受到发育调节和积累。为了寻找LIN1基因的作用和功能，我们在发育和诱导各种渗透胁迫后，在不同的亚麻组织中追踪了LIN1基因的时空表达。亚麻开花期间，LIN1 mRNA在各个器官之间差异表达。在花，茎或叶中未检测到它，但在根中发现了阳性信号。根中LIN1表达的主要位点在侧根中。通过用乙烯处理而不是通过茉莉酸甲酯或水杨酸甲酯处理，可以激活叶片中的LIN1基因。为了更好地了解其应激反应表达，我们分离了LIN1的启动子区域。对启动子区域的分析显示，它包含几个ATATT“根基序”元件和一个AGCCGCC序列，该序列在大多数基本的发病相关（PR）蛋白基因中均保守，并负责乙烯的诱导。

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  • 来源
    《International journal of plant sciences》 |2008年第6期|701-707|共7页
  • 作者

    Sabina Anzlovar; Ales Kladnik; Polona Kogovsek; Petra Nikolic; Kristina Gruden; Joze Brzin; Marina Dermastia;

  • 作者单位

    Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, SI-1000 Ljubljana, Slovenia Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, SI-1000 Ljubljana, Slovenia;

    Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, SI-1000 Ljubljana, Slovenia;

    National Institute of Biology, SI-1000 Ljubljana, Slovenia;

    Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, SI-1000 Ljubljana, Slovenia;

    National Institute of Biology, SI-1000 Ljubljana, Slovenia;

    Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, SI-1000 Ljubljana, Slovenia;

    Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, SI-1000 Ljubljana, Slovenia National Institute of Biology, SI-1000 Ljubljana, Slovenia;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    flax; in situ mRNA localization; linusitin; osmotins;

    机译：亚麻;原位mRNA定位;linusitin;渗透素;

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