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首页> 外文期刊>journal of biomedical optics >Multiphoton microscopy and fluorescence lifetime imaging provide a novel method in studying drug distribution and metabolism in the rat liver in vivo
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Multiphoton microscopy and fluorescence lifetime imaging provide a novel method in studying drug distribution and metabolism in the rat liver in vivo

机译:多光子显微镜和荧光寿命成像为研究大鼠肝脏体内药物分布和代谢提供了一种新方法

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摘要

Multiphoton microscopy has been shown to be a useful tool in studying drug distribution in biologicalntissues. In addition, fluorescence lifetime imaging provides information about the structure and dynamics of fluorophoresnbased on their fluorescence lifetimes. Fluorescein, a commonly used fluorescent probe, is metabolizednwithin liver cells to fluorescein mono-glucuronide, which is also fluorescent. Fluorescein and its glucuronide havensimilar excitation and emission spectra, but different fluorescence lifetimes. In this study, we employed multiphotonnfluorescence lifetime imaging to study the distribution and metabolism of fluorescein and its metabolite innvivo in rat liver. Fluorescence lifetime values in vitro were used to interpret in vivo data. Our results show thatnthe mean fluorescence lifetimes of fluorescein and its metabolite decrease over time after injection of fluoresceinnin three different regions of the liver. In conclusion, we have demonstrated a novel method to study a fluorescentncompound and metabolite in vivo using multiphoton fluorescence lifetime imaging.
机译:多光子显微镜已被证明是研究生物组织中药物分布的有用工具。此外,荧光寿命成像可基于荧光团的荧光寿命提供有关荧光团的结构和动力学的信息。荧光素是一种常用的荧光探针,在肝细胞内被代谢为荧光素单葡糖醛酸苷,后者也具有荧光。荧光素及其葡糖醛酸苷具有相似的激发和发射光谱,但荧光寿命不同。在这项研究中,我们采用了多光子荧光寿命成像技术来研究荧光素及其代谢产物在大鼠肝脏中的分布和代谢。体外荧光寿命值用于解释体内数据。我们的结果表明,在三个不同的肝脏区域注射荧光素后,荧光素及其代谢物的平均荧光寿命会随时间而降低。总之,我们已经证明了一种使用多光子荧光寿命成像技术在体内研究荧光化合物和代谢物的新颖方法。

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