A Simplified and Standardized Polymerase Chain Reaction Format for the Diagnosis of Leishmaniasis

Stijn Deborggraeve13 Thierry Laurent2 Diego Espinosa4 Gert Van der Auwera1 Margaret Mbuchi5 Monique Wasunna5 Sayda El-Safi6 Ahmed Almustafa Al-Basheer6 Jorge Arévalo4 César Miranda-Verástegui4 Thierry Leclipteux2 Pascal Mertens2 Jean-Claude Dujardin1 Piet Herdewijn3 and Philippe Büscher1

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A Simplified and Standardized Polymerase Chain Reaction Format for the Diagnosis of Leishmaniasis

机译：一种简化的标准化聚合酶链反应形式，用于诊断利什曼病

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Background. Definite diagnosis of Leishmania infections is based on demonstration of the parasite by microscopic analysis of tissue biopsy specimens or aspirate samples. However, microscopy generally shows low sensitivity and requires invasive sampling.

机译：背景。利什曼原虫感染的明确诊断是基于通过组织活检标本或抽吸样品的显微镜分析证实的寄生虫。但是，显微镜检查通常显示出较低的灵敏度，并且需要侵入性采样。

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《Journal of Infectious Diseases》 |2008年第10期|p.1565-1572|共8页
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Stijn Deborggraeve13 Thierry Laurent2 Diego Espinosa4 Gert Van der Auwera1 Margaret Mbuchi5 Monique Wasunna5 Sayda El-Safi6 Ahmed Almustafa Al-Basheer6 Jorge Arévalo4 César Miranda-Verástegui4 Thierry Leclipteux2 Pascal Mertens2 Jean-Claude Dujardin1 Piet Herdewijn3 and Philippe Büscher1;
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1Department of Parasitology, Institute of Tropical Medicine Antwerp, Antwerp, Belgium 2Coris BioConcept, Gembloux, Belgium 3Laboratory of Medicinal Chemistry, Rega Institute for Medicinal Research, Katholieke Universiteit Leuven, Leuven, Belgium 4Instituto de Medicina Tropical Alexander von Humboldt and Faculty of Sciences and Philosophy, Universidad Peruana Cayetano Heredia, Lima, Peru 5Center for Clinical Research, Kenya Medical Research Institute, Nairobi, Kenya 6Department of Microbiology and Parasitology, Khartoum University, Khartoum, Sudan;

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1. A simplified and standardized polymerase chain reaction format for the diagnosis of leishmaniasis. [J] . Deborggraeve S, Laurent T, Espinosa D, The Journal of Infectious Diseases . 2008,第10期

机译：一种简化的标准化聚合酶链反应形式，用于诊断利什曼病。
2. T. cruzi OligoC-TesT: A Simplified and Standardized Polymerase Chain Reaction Format for Diagnosis of Chagas Disease [J] . Stijn Deborggraeve, Ximena Coronado, Aldo Solari, PLOS Neglected Tropical Diseases . 2009,第6期

机译：T. cruzi OligoC-TesT：诊断南美锥虫病的简化和标准化聚合酶链反应形式。
3. Comparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis: diagnosis of cutaneous leishmaniasis by polymerase chain reaction. [J] . Marques MJ, Volpini AC, Machado Coelho GL, Diagnostic microbiology and infectious disease . 2006,第1期

机译：聚合酶链反应与其他实验室方法用于诊断美国皮肤利什曼病的比较：通过聚合酶链反应诊断皮肤利什曼病。
4. RAPID DIAGNOSIS OF BABESIA GIBSONI USING POINT-OF-CARE INSULATED ISOTHERMIC POLYMERASE CHAIN REACTION ASSAY [C] . Kirsten Cooke, Patrick Frenzer, Julie K. Levy, Conference of the American^College^of^Veterinary^Internal^Medicine . 2016

机译：使用护理点绝缘的等温聚合酶链反应测定快速诊断Babesia Gibsoni
5. Women's perceptions of quantitative fluorescent polymerase chain reaction technology for prenatal diagnosis [D] . Van Etten, Mara 2007

机译：妇女对产前诊断定量荧光聚合酶链反应技术的看法
6. A Simplified and Standardized Polymerase Chain Reaction Format for the Diagnosis of Leishmaniasis [O] . Stijn Deborggraeve, Thierry Laurent, Diego Espinosa, -1

机译：一种简化的标准化聚合酶链反应形式用于诊断利什曼病
7. A Simplified and Standardized Polymerase Chain Reaction Format for the Diagnosis of Leishmaniasis [O] . 2015

机译：用于诊断利什曼病的简化和标准化聚合酶链反应形式
8. Services to Develop, Standardize, and Validate Polymerase Chain Reaction (PCR)Protocols for the Detection of Leishmaniasis in Clinical Samples [R] . White, F. A., Suryanaarayana, K. 1994

机译：开发，标准化和验证聚合酶链式反应（pCR）协议的服务，用于检测临床样本中的利什曼病

A Simplified and Standardized Polymerase Chain Reaction Format for the Diagnosis of Leishmaniasis

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