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首页> 外文期刊>Japanese journal of applied physics >In situ observation of containerless protein crystallization by magnetically levitating crystal growth
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In situ observation of containerless protein crystallization by magnetically levitating crystal growth

机译：通过磁悬浮晶体生长原位观察无容器蛋白质的结晶

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摘要

We report on the results of the crystal growth of hen-egg lysozyme by magnetically levitating crystals in a small amount of buffer solution. The concentrations of lysozyme and the precipitating agent (gadolinium chloride) were 6.53wt% and 0.362 mol/kg, respectively. Gadolinium chloride, which induces the magneto-Archimedes effect, was utilized to levitate the crystals with B-z.(dB(z)/dz) = 22.46 T-2/m, where B-z is the vertical (z) component of the magnetic flux density vector. Although the collected crystals were small, we succeeded in maintaining the levitation of the crystals into a specific place in the liquid phase from the beginning of nucleation. In situ observation revealed that a state of pseudo-weightlessness was generated in the vicinity of the magnet bore edge, and small crystals were concentrated inside the domain moving along an hourglass-shaped surface. We found by numerical computations that the formation of the hourglass-shaped domain is attributable to the radial component of the magnetic force. (C) 2016 The Japan Society of Applied Physics
机译：我们报告了通过在少量缓冲液中磁悬浮晶体使鸡蛋卵溶菌酶晶体生长的结果。溶菌酶和沉淀剂（氯化ga）的浓度分别为6.53wt％和0.362mol / kg。氯化诱导磁-阿基米德效应，被用来悬浮Bz的晶体。（dB（z）/ dz）= 22.46 T-2 / m，其中Bz是磁通密度的垂直（z）分量向量。尽管收集的晶体很小，但我们从成核开始就成功地将晶体悬浮在液相中的特定位置。原位观察表明，在磁铁孔边缘附近产生了伪失重状态，并且小晶体集中在沿沙漏形表面移动的畴内。通过数值计算我们发现，沙漏形区域的形成可归因于磁力的径向分量。 （C）2016年日本应用物理学会

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    《Japanese journal of applied physics》 |2016年第3期|035505.1-035505.6|共6页
  • 作者

    Maki Syou; Tanimoto Yoshifumi; Udagawa Chikako; Morimoto Shotaro; Hagiwara Masayuki;

  • 作者单位

    Osaka Ohtani Univ, Fac Pharm, Lab Mol Chem, Tondabayashi, Osaka 5848540, Japan;

    Osaka Ohtani Univ, Fac Pharm, Lab Mol Chem, Tondabayashi, Osaka 5848540, Japan;

    Osaka Ohtani Univ, Fac Pharm, Lab Mol Chem, Tondabayashi, Osaka 5848540, Japan;

    Osaka Ohtani Univ, Fac Pharm, Lab Mol Chem, Tondabayashi, Osaka 5848540, Japan;

    Osaka Univ, Grad Sch Sci, Ctr Adv High Magnet Field Sci, Toyonaka, Osaka 5600043, Japan;

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