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Long period preservation of marine products using electrostatic field

机译：利用静电场长期保存海产品

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The effect of an AC electric field on the freshness of marine products was experimentally investigated. An AC voltage of 10 kV with 50Hz in frequency was generated with a transformer and applied to a plane electrode set in an incubator. The biological material was the gonad of purple sea urchin. The AC electric field with 50Hz in frequency was applied to the gonad at -1 degrees C for 7 days. Freshness was evaluated by measuring protein release and lactate dehydrogenase (LDH) activity. The results showed that the protein release and LDH activity in the gonad were suppressed by applying the AC electric field, compared with that without the AC electric field. Moreover, the gonad was treated with the AC electric field at -5 degrees C for 3 days and then preserved at 0 degrees C for 1 day without the field. This electrical field treatment of the gonad prolonged the freshness date for more than 10 days under 4 degrees C preservation condition. In addition, the permeability of the cell membrane was suppressed by applying the AC electric field. Concerning the relationship between permeability and AC electric field, the conformational change of bovine serum albumin (BSA) induced by the AC electric field was evaluated on the basis of UVabsorption spectra. The results revealed that the secondary and/or higherorder structure gradually changes with preservation period. The conformational change of the BSA molecule was induced by applying the AC electric field. (C) 2016 The Japan Society of Applied Physics
机译：实验研究了交流电场对海产品新鲜度的影响。用变压器产生频率为50Hz的10 kV交流电压，并将其施加到设置在培养箱中的平面电极上。生物材料是紫海胆的性腺。将频率为50Hz的交流电场在-1摄氏度的性腺上施加7天。通过测量蛋白质释放和乳酸脱氢酶（LDH）活性来评估新鲜度。结果表明，与没有交流电场的情况相比，施加交流电场可以抑制性腺中的蛋白质释放和LDH活性。此外，将性腺用-5℃的AC电场处理3天，然后在0℃下保存1天而无电场。这种性腺的电场处理在4°C的保存条件下将新鲜日期延长了10天以上。另外，通过施加交流电场抑制了细胞膜的渗透性。关于渗透率和交流电场之间的关系，基于紫外线吸收光谱，评估了由交流电场引起的牛血清白蛋白（BSA）的构象变化。结果表明，二级和/或高级结构随着保存期的变化而逐渐变化。通过施加交流电场来诱导BSA分子的构象变化。 （C）2016年日本应用物理学会

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  • 来源
    《Japanese journal of applied physics》 |2016年第7s2期|07LG07.1-07LG07.4|共4页
  • 作者

    Okumura Takamasa; Yaegashi Taro; Yamada Kazuki; Ito Takanori; Takahashi Katsuyuki; Aisawa Sumio; Takaki Koichi; Yamazaki Shigeyoshi; Syuto Bunei;

  • 作者单位

    Iwate Univ, Dept Elect Engn & Comp Sci, Morioka, Iwate 0208551, Japan;

    Iwate Univ, Dept Elect Engn & Comp Sci, Morioka, Iwate 0208551, Japan;

    Iwate Univ, Dept Elect Engn & Comp Sci, Morioka, Iwate 0208551, Japan;

    Iwate Univ, Dept Elect Engn & Comp Sci, Morioka, Iwate 0208551, Japan;

    Iwate Univ, Dept Elect Engn & Comp Sci, Morioka, Iwate 0208551, Japan|Iwate Univ, Soft Path Engn Res Ctr SPERC, Morioka, Iwate 0208551, Japan;

    Iwate Univ, Dept Elect Engn & Comp Sci, Morioka, Iwate 0208551, Japan|Iwate Univ, Soft Path Engn Res Ctr SPERC, Morioka, Iwate 0208551, Japan;

    Iwate Univ, Dept Elect Engn & Comp Sci, Morioka, Iwate 0208551, Japan|Iwate Univ, Soft Path Engn Res Ctr SPERC, Morioka, Iwate 0208551, Japan;

    Iwate Univ, Ctr Reg Collaborat Res & Educ, Morioka, Iwate 0208551, Japan;

    Iwate Univ, Ctr Reg Collaborat Res & Educ, Morioka, Iwate 0208551, Japan;

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