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首页> 外文期刊>Japanese journal of applied physics >Electrokinetic Evaluation of Individual Exosomes by On-Chip Microcapillary Electrophoresis with Laser Dark-Field Microscopy
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Electrokinetic Evaluation of Individual Exosomes by On-Chip Microcapillary Electrophoresis with Laser Dark-Field Microscopy

机译：芯片上微毛细管电泳和激光暗场显微镜对单个外泌体的电动评估

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摘要

Cell-secreted nanovesicles called exosomes are expected as a promising candidate biomarker of various diseases. Toward the future application of exosomes as a disease biomarker for low-invasive diagnostics, challenges remain in the development of sensitive and precise analysis methods for exosomes. In this study, we performed the electrokinetic evaluation of individual exosomes by the combined use of on-chip microcapillary electrophoresis and laser dark-field microscopy. We extracted exosomes from six types of human cell cultured in a serum-free medium by differential ultracentrifugation and their zeta potential (electrophoretic mobility) were evaluated. We demonstrated that the proposed electrophoresis apparatus is particularly suitable for the tracking analysis of the electrophoretic migration of individual exosomes and enables the accurate evaluation of the zeta potential distribution of exosomes, for the first time. From the experimental results, we found that there is a strong correlation between the average zeta potentials of exosomes and their cells of origin.
机译：细胞分泌的纳米囊泡称为外泌体，有望成为各种疾病的有前途的候选生物标志物。未来将外泌体用作低侵入性诊断的疾病生物标志物，开发外泌体的灵敏，精确的分析方法仍面临挑战。在这项研究中，我们通过芯片上微毛细管电泳和激光暗场显微镜的结合使用对单个外泌体进行了电动评估。我们通过超速离心从无血清培养基中培养的六种人类细胞中提取了外泌体，并评估了它们的Zeta电位（电泳迁移率）。我们证明了所提出的电泳设备特别适用于单个外泌体电泳迁移的追踪分析，并首次实现了对外泌体的ζ电势分布的准确评估。从实验结果中，我们发现外泌体的平均Zeta电位与其来源细胞之间存在很强的相关性。

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  • 来源
    《Japanese journal of applied physics》 |2013年第6issue2期|06GK10.1-06GK10.4|共4页
  • 作者

    Kei Kato; Masashi Kobayashi; Nami Hanamura; Takanori Akagi; Nobuyoshi Kosaka; Takahiro Ochiya; Takanori Ichiki;

  • 作者单位

    Department of Bioengineering, School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan;

    Department of Bioengineering, School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan;

    Department of Bioengineering, School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan;

    Department of Bioengineering, School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan;

    Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Chuo, Tokyo 104-0045, Japan;

    Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Chuo, Tokyo 104-0045, Japan;

    Department of Bioengineering, School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan;

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