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首页> 外文期刊>Japanese journal of applied physics >Subharmonic Synchronization of Picosecond Yb Fiber Laser to Picosecond TkSapphire Laser for Stimulated Raman Scattering Microscopy
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Subharmonic Synchronization of Picosecond Yb Fiber Laser to Picosecond TkSapphire Laser for Stimulated Raman Scattering Microscopy

机译：皮秒Yb光纤激光器与皮秒Tk蓝宝石激光器的次谐波同步，用于受激拉曼散射显微镜。

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摘要

We demonstrate a subharmonically synchronized picosecond laser system and its application to stimulated Raman scattering (SRS) microscopy for label-free biological imaging with high sensitivity and high spectral resolution. Experimentally, 5.5-ps Yb fiber laser pulses at a repetition rate of 38 MHz were successfully synchronized to 4.8-ps TkSapphire laser pulses at a repetition rate of 76 MHz, by using a two-photon detector, an intracavity electro-optic modulator, and a piezoelectric transducer. The temporal jitter was measured to be 120fs. These pulses were applied to SRS microscopy. We confirmed the shot-noise-limited sensitivity, and succeeded in SRS spectroscopy of a polymer bead as well as three-dimensional SRS imaging of a HeLa cell.
机译：我们演示了亚谐同步皮秒激光系统及其在受激拉曼散射（SRS）显微镜中用于无标记生物成像的高灵敏度和高光谱分辨率的应用。在实验中，通过使用双光子检测器，腔内电光调制器，以及以38 MHz的重复频率成功将5.5 ps的Yb光纤激光脉冲与以76 MHz的重复频率进行同步的4.8 ps TkSapphire激光脉冲进行了同步。压电换能器。经测量，时间抖动为120fs。这些脉冲被应用于SRS显微镜。我们确认了散粒噪声限制的灵敏度，并成功地完成了聚合物珠粒的SRS光谱以及HeLa细胞的三维SRS成像。

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  • 来源
    《Japanese journal of applied physics》 |2012年第2issue1期|p.022702.1-022702.4|共4页
  • 作者

    Wataru Umemura; Kenta Fujita; Yasuyuki Ozeki; Ken Goto; Kazuhiko Sumimura; Norihiko Nishizawa; Kiichi Fukui; Kazuyoshi Itoh;

  • 作者单位

    Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan;

    Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan;

    Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan,PRESTO, Japan Science and Technology Agency (JST), Kawaguchi, Saitama 332-0012, Japan;

    Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan;

    Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan;

    Department of Electrical Engineering and Computer Science, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan;

    Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan;

    Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan;

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