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首页> 外文期刊>Japanese journal of applied physics >Improvement of Electrical Stimulation Protocol for Simultaneous Measurement of Extracellular Potential with On-Chip Multi-Electrode Array System
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Improvement of Electrical Stimulation Protocol for Simultaneous Measurement of Extracellular Potential with On-Chip Multi-Electrode Array System

机译：片上多电极阵列系统用于同时测量细胞外电位的电刺激方案的改进

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摘要

Cardiotoxicity testing with a multi-electrode array (MEA) system requires the stable beating of cardiomyocytes for the measurement of the field potential duration (FPD), because different spontaneous beating rates cause different responses of FPD prolongation induced by drugs, and the beating rate change effected by drugs complicates the FPD prolongation assessment. We have developed an on-chip MEA system with electrical stimulation for the measurement of the FPD during the stable beating of human embryonic stern (ES) cell-derived cardiomyocyte clusters. Using a conventional bipolar stimulation protocol, we observed such large artifacts in electrical stimulation that we could not estimate the FPD quantitatively. Therefore, we improved the stimulation protocol by using sequential rectangular pulses in which the positive and negative stimulation voltages and number of pulses could be changed flexibly. The balanced voltages and number of pulses for sequential rectangular, pulses enabled the recording of small negative artifacts only, which hardly affected the FPD measurement of human-ES-cell-derived cardiomyocyte clusters. These conditions of electrical stimulation are expected to find applications for the control of constant beating for cardiotoxicity testing.
机译：使用多电极阵列（MEA）系统进行的心脏毒性测试需要稳定的跳动心肌细胞来测量场电位持续时间（FPD），因为不同的自发搏动速率会导致药物诱导的FPD延长反应不同，并且搏动速率会发生变化受药物影响会使FPD延长评估变得复杂。我们已经开发了一种带电刺激的片上MEA系统，用于在人胚（ES）细胞衍生的心肌细胞簇稳定跳动期间测量FPD。使用常规的双极刺激方案，我们在电刺激中观察到如此大的伪像，因此无法定量估计FPD。因此，我们通过使用顺序矩形脉冲改进了刺激方案，在该矩形脉冲中可以灵活地更改正负刺激电压和脉冲数。连续矩形脉冲的平衡电压和脉冲数仅能记录小的负伪像，这几乎不会影响人ES细胞衍生的心肌细胞簇的FPD测量。预期这些电刺激条件可找到用于控制心脏毒性测试的持续搏动的应用。

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  • 来源
    《Japanese journal of applied physics》 |2012年第6issue2期|p.06FK02.1-06FK02.5|共5页
  • 作者

    Tomoyuki Kaneko; Fumimasa Nomura; Akihiro Hattori; Kenji Yasuda;

  • 作者单位

    Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University,Chiyoda, Tokyo 101-0062, Japan;

    Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University,Chiyoda, Tokyo 101-0062, Japan;

    Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University,Chiyoda, Tokyo 101-0062, Japan;

    Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University,Chiyoda, Tokyo 101-0062, Japan;

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