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首页> 外文期刊>Japanese journal of applied physics.Part 1.Regular papers & short notes >High-Speed Imaging of Broadband Multiplex Coherent Anti-Stokes Raman Scattering Microscopy Using a Supercontinuum Source
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High-Speed Imaging of Broadband Multiplex Coherent Anti-Stokes Raman Scattering Microscopy Using a Supercontinuum Source

机译:使用超连续谱源的宽带多路相干反斯托克斯拉曼散射显微镜的高速成像

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摘要

We combined broadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy with CARS microscopy using a femtosecond laser and a photonic crystal fiber. Broadband multiplex CARS microspectroscopy, which simultaneously measures broadband range (including significant Raman vibrational modes) from 500 to 3500 cm~-1 at a high spectral resolution was subsequently performed. Also, via multiplex CARS microscopy using a pair of galvanometer mirrors we successfully demonstrated the high-speed imaging of polymer beads and a lily pollen grain for the study of cell biology dynamics. The imaging speed was 37 s per image, which is 30-40 times faster than that previously reported in broadband CARS microscopy.
机译:我们将飞秒激光和光子晶体光纤与宽带多路相干相干抗斯托克斯拉曼散射(CARS)显微技术与CARS显微镜相结合。随后进行了宽带多路复用CARS显微技术,该技术以高光谱分辨率同时测量了500至3500 cm〜-1的宽带范围(包括重要的拉曼振动模式)。此外,通过使用一对振镜的多重CARS显微镜,我们成功地展示了聚合物珠和百合花粉粒的高速成像技术,用于研究细胞生物学动力学。每个图像的成像速度为37 s,比以前在宽带CARS显微镜中报道的速度快30-40倍。

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    School of Photon Science and Technology, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea;

    School of Photon Science and Technology, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea;

    Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea;

    Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea;

    Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea;

    Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea;

    School of Photon Science and Technology, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea,Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea;

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