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首页> 外文期刊>Japanese journal of applied physics >Liquid Phase Immunoassay Using Magnetic Markers and Superconducting Quantum Interference Device
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Liquid Phase Immunoassay Using Magnetic Markers and Superconducting Quantum Interference Device

机译：磁性标记和超导量子干涉仪的液相免疫分析

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摘要

A liquid phase immunoassay utilizing magnetic markers and a high-T_c superconducting quantum interference device (SQUID) was studied. In this method, the biological target is detected using magnetic markers, i.e., the magnetic signal from the markers that bound to the target is detected with the SQUID. The detection was performed in a solution containing both the bound and unbound (free) markers without using the so-called bound/free (BF) separation process. The bound markers were distinguished from the free markers by utilizing the Brownian rotation of the free markers. First, the properties of the free markers in the solution, such as the M-H curve and magnetic relaxation, were measured to study the background signal from the free markers. Markers that exhibit remanence were used for the experiment. Using the obtained results, we discuss the effects of the residual earth field and aggregation of the markers on the background signal. Next, we detected a fungus, Candida albicans, with the described liquid phase immunoassay. Good relationship was obtained between the detected signal and the number of fungi. The minimum detectable number of fungi was as small as 30.
机译：研究了利用磁性标记和高T_c超导量子干涉仪（SQUID）的液相免疫测定。在该方法中，使用磁性标记物检测生物靶标，即，用SQUID检测来自与靶标结合的标记物的磁信号。在包含结合和未结合（游离）标记的溶液中进行检测，而无需使用所谓的结合/游离（BF）分离过程。通过利用自由标记的布朗旋转，将结合的标记与自由标记区分开。首先，测量溶液中游离标记的性质，例如M-H曲线和磁弛豫，以研究来自游离标记的背景信号。表现出剩磁的标记用于实验。使用获得的结果，我们讨论了残留地球场和标记聚集对背景信号的影响。接下来，我们用所述的液相免疫测定法检测了一种真菌白色念珠菌。在检测到的信号和真菌数量之间获得了良好的关系。真菌的最小可检测数量只有30个。

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