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首页> 外文期刊>Japanese Journal of Applied Physics. Part 1, Regular Papers, Brief Communications & Review Papers >Alignment of λ-DNA on Organic Monolayer Surface Patterned by Scanning Probe Lithography
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Alignment of λ-DNA on Organic Monolayer Surface Patterned by Scanning Probe Lithography

机译：扫描探针光刻法在有机单层表面上对λ-DNA的比对

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摘要

With the combination of a molecular combing technique and scanning probe lithographic patterning, λ-DNA wires were stretched and aligned to form parallel lines on a patterned organic monolayer surface. The patterned organic monolayer was composed of hydrophilic aminopropyltriethoxysilane (APS) in the middle of hydrophobic octadecyltrichlorosilane (OTS) on a silicon surface. The stretching and alignment of λ-DNA wires were induced along the lines of the APS layer which attracted λ-DNA through a coulombic interaction at an early stage of the combing. Weaker van der Waals interaction with the OTS layer induced a settlement of the wire after the sample was dried. The aligned structures were confirmed by tapping-mode atomic force microscope images, aided by positively-charged gold nanoparticles exclusively attached to the DNA backbone.
机译：结合分子梳理技术和扫描探针光刻图案，将λ-DNA线拉伸并对齐以在图案化的有机单层表面上形成平行线。图案化的有机单层由亲水性氨基丙基三乙氧基硅烷（APS）组成，位于硅表面的疏水性十八烷基三氯硅烷（OTS）的中间。沿APS层的线诱导λ-DNA线的拉伸和排列，该APS层在精梳初期通过库伦相互作用吸引λ-DNA。样品干燥后，较弱的范德华斯与OTS层的相互作用导致金属丝沉降。排列的结构通过敲击模式原子力显微镜图像得到了证实，借助仅附着在DNA主链上的带正电的金纳米颗粒。

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