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首页> 外文期刊>Japanese Journal of Applied Physics. Part 1, Regular Papers, Brief Communications & Review Papers >Detection of Streptavidin-Biotin Protein Complexes Using Three-Dimensional MOSFET in the Si Micro-Fluidic Channel
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Detection of Streptavidin-Biotin Protein Complexes Using Three-Dimensional MOSFET in the Si Micro-Fluidic Channel

机译：在硅微流体通道中使用三维MOSFET检测链霉亲和素-生物素蛋白复合物

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摘要

To detect the electrical reaction characteristics of streptavidin-biotin protein complexes, a three-dimensional (3-D) p-channel metal oxide semiconductor field effect transistor (PMOSFET)-type biosensor was fabricated in the convex corner of a micro-fluidic channel by tetramethyl ammonium hydroxide (TMAH) anisotropic etching. Au, which has a chemical affinity with thiol, was used as the gate metal for immobilizing a self-assembled monolayer (SAM). The SAM was used to immobilize streptavidin. The hydroxyl group of SAM was bound with the amine group of streptavidin. After that, streptavidin and biotin were bound by their high affinity (Ka ～ 10~(15) Mol~(-1)). The measurements were performed in phosphate-buffered saline (PBS; pH 6.4, 20 mM) solution. Ag/AgCl was used as the reference electrode. The bindings of SAM, streptavidin and biotin caused a variation in the drain current of the 3-D MOSFET. To verify the interaction among the SAM, streptavidin and biotin, surface plasmon resonance (SPR) measurement was performed.
机译：为了检测链霉亲和素-生物素蛋白复合物的电反应特性，在微流体通道的凸角处制作了三维（3-D）p沟道金属氧化物半导体场效应晶体管（PMOSFET）型生物传感器。四甲基氢氧化铵（TMAH）各向异性蚀刻。与硫醇具有化学亲和力的金被用作固定自组装单分子层（SAM）的栅极金属。 SAM用于固定链霉亲和素。 SAM的羟基与链霉亲和素的胺基结合。此后，链霉亲和素和生物素以其高亲和力（Ka〜10〜（15）Mol〜（-1））结合。测量在磷酸盐缓冲盐水（PBS； pH 6.4，20 mM）溶液中进行。将Ag / AgCl用作参比电极。 SAM，链霉亲和素和生物素的结合导致3-D MOSFET的漏极电流发生变化。为了验证SAM，链霉亲和素和生物素之间的相互作用，进行了表面等离振子共振（SPR）测量。

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