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首页> 外文期刊>Journal of the American Oil Chemists' Society >Lipase-Catalyzed Acidolysis of Olive Oil with Echium Oil Stearidonic Acid: Optimization by Response Surface Methodology
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Lipase-Catalyzed Acidolysis of Olive Oil with Echium Oil Stearidonic Acid: Optimization by Response Surface Methodology

机译:脂肪酶催化的油和硬脂酸硬脂酸对橄榄油的酸解:通过响应面法进行优化

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摘要

In this study, our aim was to enrich olive oil with stearidonic acid (SDA) together with polyunsaturated fatty acids (PUFA) by lipase-catalyzed acidolysis using olive oil (OO) and free fatty acids of Echium oil, in the presence of Lipozyme® TL IM. The reaction conditions were optimized by using response surface methodology. A three-factor, five level central composite circumscribed designs was used to generate the design points. The factors chosen were: substrate molar ratio (S r, 4–6 mol/mol), reaction temperature (T, 55–65 °C), and reaction time (t, 6–9 h). Targeted incorporation (5 %) of SDA into OO was achieved at substrate molar ratio of 6 mol/mol, 55 °C, and 8.4 h. Model verification performed under these conditions for mg- and g-scale production yielded SDA contents of 4.9 and 4.8 %, respectively. Moreover, it was observed that the structured lipid (SL) obtained under optimum conditions contained approximately 42 % oleic acid and 43 % PUFA including linoleic acid, α-linolenic acid, and γ-linolenic acid. Omega-6/omega-3 fatty acid ratio of SLs was 0.7. Analysis of oxidative properties resulted in lower oxidative stability of SL than unmodified OO. This type of SL containing SDA and other PUFA is believed to be beneficial for human health.
机译:在这项研究中,我们的目的是在存在Lipozyme®的情况下,利用橄榄油(OO)和Echium油中的游离脂肪酸,通过脂肪酶催化的酸解作用,使橄榄油中的硬脂酸(SDA)与多不饱和脂肪酸(PUFA)一起富集。 TL IM。通过使用响应面法优化了反应条件。使用三因素,五级中央组合外接设计来生成设计点。选择的因素为:底物摩尔比(S r ,4–6 mol / mol),反应温度(T,55–65°C)和反应时间(t,6–9 h)。在6 mol / mol,55°C和8.4 h的底物摩尔比下,将SDA定向掺入OO中(5%)。在这些条件下进行的毫克级和克级生产模型验证分别得出SDA含量为4.9%和4.8%。此外,观察到在最佳条件下获得的结构化脂质(SL)包含约42%的油酸和43%的PUFA,包括亚油酸,α-亚麻酸和γ-亚麻酸。 SL的Omega-6 / omega-3脂肪酸比率为0.7。氧化性能的分析导致SL的氧化稳定性低于未改性的OO。据信这种类型的含有SDA和其他PUFA的SL对人体健康有益。

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