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首页> 外文期刊>Journal of Analytical Atomic Spectrometry >Morphology and characterization of Dematiaceous fungi on a cellulose paper substrate using synchrotron X-ray microtomography, scanning electron microscopy and confocal laser scanning microscopy in the context of cultural heritage
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Morphology and characterization of Dematiaceous fungi on a cellulose paper substrate using synchrotron X-ray microtomography, scanning electron microscopy and confocal laser scanning microscopy in the context of cultural heritage

机译：在文化遗产背景下，使用同步加速器X射线显微断层照相术，扫描电子显微镜和共聚焦激光扫描显微镜对纤维素纸基质上的脱皮真菌进行形态学和表征

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摘要

Dematiaceous (black pigmented) fungi interact with a cellular paper matrix growing on the surface and in paper bulk. Fungi-induced stains are one form of such interactions and are referred to as biodeterioration when applied to cultural heritage materials. The complexity of both paper and living systems, such as fungi, requires multi-scale analysis. The surface topography and spatial distribution of fungal monilioid hyphae, branched and un-branched chains and morphology of isodiametric enlargements of spores and yeast-like cells on the surface of paper were imaged by correlative microscopy, combining environmental scanning electron microscopy and imaging in backscattered electron mode (SEM-BSE) with confocal laser scanning microscopy (CLSM). The spherical fruiting bodies (perithecia) embedded in paper were not detected by CLSM, and ESEM provided data collected near the surface. Their interaction with paper was analyzed by 3D visualization using X-ray microtomography (XμCT). Until now the fungi and paper interfaces in the matrix (bulk) of paper have not been analyzed using XμCT. We present a novel method for investigating the interaction of fungal pigmented mycelia, spores and perithecia in the paper matrix using XμCT X and 3D visualization based on the XμCT data. The tomographs were generated on the designated ID19 beam at ESRF, Grenoble, France. The ultimate purpose of this investigation was to understand the mechanisms of fungi and paper interactions in order to develop preservation strategies for cultural heritage, such as historic and artistic works on paper infested by fungi.
机译：硬皮病（黑色色素）真菌与在表面和纸团中生长的细胞纸基质相互作用。真菌引起的污渍是这种相互作用的一种形式，当应用于文化遗产材料时，被称为生物退化。纸张和生命系统（例如真菌）的复杂性都需要多尺度分析。通过相关显微镜，结合环境扫描电子显微镜和背散射电子成像，对纸张表面真菌单核菌丝菌丝，支链和非支链的表面形貌和空间分布以及孢子和酵母样细胞等径扩大的形态进行了成像。共聚焦激光扫描显微镜（CLSM）扫描模式（SEM-BSE）。 CLSM未检测到嵌入纸中的球形子实体（皮膜），而ESEM提供了在表层附近收集的数据。使用X射线显微断层扫描（XμCT）通过3D可视化分析了它们与纸张的相互作用。到目前为止，尚未使用XμCT分析纸基质（散装）中的真菌和纸界面。我们提出了一种新的方法，用于研究使用XμCTX和基于XμCT数据的3D可视化在纸质基质中的真菌色素菌丝体，孢子和皮膜的相互作用。断层扫描仪是在法国格勒诺布尔的ESRF的指定ID19光束上生成的。这项调查的最终目的是了解真菌和纸张相互作用的机制，以便制定文化遗产的保护策略，例如在真菌感染下的纸张上的历史和艺术作品。

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  • 来源
    《Journal of Analytical Atomic Spectrometry》 |2015年第3期|651-657|共7页
  • 作者

    H. M. Szczepanowska; D. Jha; Th. G. Mathia;

  • 作者单位

    Museum Conservation Institute, Smithsonian Institution, Suitland, MD, USA;

    Nano-Science Center, Department of Chemistry, University of Copenhagen, Copenhagen, Denmark;

    C.N.R.S. Ecole Centrale de Lyon, Laboratoire de Tribologie et Dynamique des Systemese, France;

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  • 入库时间 2022-08-18 03:04:06

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