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首页> 外文期刊>Journal of Analytical Atomic Spectrometry >To shift, or not to shift: adequate selection of an internal standard in mass-shift approaches using tandem ICP-mass spectrometry (ICP-MS/MS)
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To shift, or not to shift: adequate selection of an internal standard in mass-shift approaches using tandem ICP-mass spectrometry (ICP-MS/MS)

机译：使用串联ICP - 质谱（ICP-MS / MS）来转移或不转移：充分选择大规模换档方法中的内部标准

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摘要

The use of an internal standard to correct for potential matrix effects and instrument instability is common practice in ICP-MS. However, with the introduction of a new generation of ICP-MS instrumentation with a tandem mass spectrometry configuration (ICP-MS/MS), the use of chemical resolution in a mass-shift approach has become much more popular, suggesting that the appropriate selection of an internal standard needs revision. In this particular case, it needs to be decided whether the internal standard should also be subjected to a mass-shift or can simply be monitored on-mass ("to shift, or not to shift"). In this work, 17 elements covering a wide range of masses (24-205 amu) and ionization energies (3.89-9.39 eV) were measured via on-mass and/or mass-shift strategies, and the corresponding atomic ions and reaction product ions were monitored during various systematic experiments. For mass-shifting, an NH_3/He gas mixture was used to obtain NH_3-based reaction product ions (cluster formation). Product ion scanning (PIS) was used for assessing the differences in reactivity between the different analytes and for the identification of the best suited reaction product ions. It was found that the use of chemical resolution can significantly affect the short-term signal stability and that ion signals measured on-mass are not affected in the same way as those measured mass-shifted. Variations affecting the signal intensities of both atomic and reaction product ions can be attributed to the ion-molecule chemistry occurring within the collision/reaction cell and were found to be related with some degree of initial instability in the cell and differences in reactivity. The use of a sufficiently long stabilization time, however, avoids or at least mitigates such differences in the behavior between signals monitored on-mass and after mass-shifting, respectively. Furthermore, the introduction of cell disturbances, such as those generated after quickly switching between different sets of operating conditions in a multi-tune method, revealed significant differences in signal behavior between atomic and reaction product ions, potentially hampering the use of an internal standard monitored on-mass when the analysis is based on an analyte monitored after mass-shifting. However, the use of a reasonable waiting time again greatly mitigates such differences, with the duration of this stabilization time depending on the magnitude of the cell disturbances (e.g., switch between vented and pressurized mode or only between pressurized modes using different gas flow rates). In addition, also the effect of varying different instrument settings (plasma power, torch position, and gas and liquid flow rates) was evaluated, but no remarkable differences were found between signals monitored on-mass and those mass-shifted. Interestingly, a statistical evaluation of the influence of the different settings on the signal intensities of all ions monitored did not reveal the a priori important role of some properties traditionally suggested for adequate selection of analyte/internal standard pairs, such as mass number or ionization energy, as also suggested in other recent studies.
机译：使用内标以纠正潜在的矩阵效应和仪器不稳定性是ICP-MS中的常见做法。但是，随着新一代ICP-MS仪表具有串联质谱配置（ICP-MS / MS），在大规模换档方法中使用化学分辨率已经变得更加流行，表明适当的选择内部标准需要修订。在这种特殊情况下，需要确定内部标准是否应受到大规模转移，或者可以简单地监测质量（“转移或不转移”）。在这项工作中，通过质量和/或大规模换档策略测量覆盖各种质量范围（24-205AMU）和电离能量（3.89-9.39eV）的17个元素，以及相应的原子离子和反应产物离子在各种系统实验期间监测。为了大规模转移，使用NH_3 / HE气体混合物来获得基于NH_3的反应产物离子（簇形成）。产品离子扫描（PIS）用于评估不同分析物之间的反应性的差异，并鉴定最适合的反应产物离子。发现使用化学分辨率可以显着影响短期信号稳定性，并且测量的离子信号在质量上不受相同的方式影响，因为测量的质量移位。影响原子和反应产物离子的信号强度的变型可归因于碰撞/反应细胞内发生的离子分子化学，并且发现与细胞中的一定程度的初始不稳定性有关，并且反应性的差异有关。然而，使用足够长的稳定时间，避免了或至少减轻了在质量受到质量的信号之间的行为之间的这种差异。此外，引入细胞扰动，例如在多曲程方法中快速切换后产生的细胞紊乱，在多曲程法中快速切换，揭示了原子和反应产物离子之间的信号行为的显着差异，可能阻碍了监测内部标准的使用当分析基于大规模化后监测的分析物时质量。然而，使用合理的等待时间再次大大减轻这种差异，这是根据电池干扰的幅度的持续时间（例如，使用不同的气流之间或仅在加压模式之间切换或仅在加压模式之间切换） 。此外，还评估了不同仪器设置（等离子体动力，割炬位置和气体流量和液体流速）的效果，但在受到质量监测的信号之间没有发现显着的差异，并且这些偏移。 Interestingly, a statistical evaluation of the influence of the different settings on the signal intensities of all ions monitored did not reveal the a priori important role of some properties traditionally suggested for adequate selection of analyte/internal standard pairs, such as mass number or ionization energy如其他最近的研究所述。

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  • 来源
    《Journal of Analytical Atomic Spectrometry》 |2021年第6期|1135-1149|共15页
  • 作者

    Eduardo Bolea-Fernandez; Ana Rua-lbarz; Martín Resano; Frank Vanhaecke;

  • 作者单位

    Ghent University Department of Chemistry Atomic & Mass Spectrometry - A&MS research group Campus Sterre Krijgslaan 281-S12 9000 Ghent Belgium;

    Ghent University Department of Chemistry Atomic & Mass Spectrometry - A&MS research group Campus Sterre Krijgslaan 281-S12 9000 Ghent Belgium;

    University of Zaragoza Aragón Institute of Engineering Research (13A) Department of Analytical Chemistry Pedro Cerbuna 12 50009 Zaragoza Spain;

    Ghent University Department of Chemistry Atomic & Mass Spectrometry - A&MS research group Campus Sterre Krijgslaan 281-S12 9000 Ghent Belgium;

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