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首页> 外文期刊>Journal of apicultural science >INFLUENCE OF THE AGE OF HONEY BEE QUEENS AND DOSE OF SEMEN ON CONDITION OF INSTRUMENTALLY INSEMINATED QUEENS KEPT IN CAGES WITH 25 WORKER BEES IN BEE COLONIES
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INFLUENCE OF THE AGE OF HONEY BEE QUEENS AND DOSE OF SEMEN ON CONDITION OF INSTRUMENTALLY INSEMINATED QUEENS KEPT IN CAGES WITH 25 WORKER BEES IN BEE COLONIES

机译：蜂群中25只工蜂中蜂蜜蜂皇后的年龄和精液剂量对无症状昆士兰保存的条件的影响

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The study was done in the Department of Bee Breeding in the Institute of Pomology and Floriculture, Apiculture Division in Pulawy, Poland. It was done in July and August of 2006 and 2007. The aim of the research was to determine the age of queens when inseminated and the dose of semen used in insemination. The condition of the oviducts and the filling of the spermatheca were determined. Queens 5, 7 or 10 days old were inseminated with 6, 8, or 10 ui of semen collected from free flying Caucasian drones. In both years, 1027 queens were inseminated and dissected. Altogether 80.3% of inseminated queens had cleared oviducts, 16.6% had some residue of semen, and 3.1% died. Significantly more queens which were inseminated at the age of 7 and 10 days had cleared the oviducts (82.3 and 86.2% respectively) compared to 5-day-old ones (72.4%). Among the 5 days old, 23.8% queens had some residue of semen. The losses of queens 5, 7 and 10 day-old were 48 hours after insemination similar (3.8, 3.2 and 2.3% respectively).rnQueens that cleared their oviducts had on an average 3.199 mln spermatozoa in the spermatheca. A significantly higher number of spermatozoa in the spermatheca (average 3.4 mln) was found in a group of queens that were inseminated at the age of 5 days. A lower number of spermatozoa was found in queens that were inseminated at the age of 7 and 10 days, regardless of the dose of semen. It was stated that most of the queens had from 3 mln to 4.5 mln of spermatozoa in their spermatheca. The highest percent of queens that had more than 4.5 mln of spermatozoa in their spermatheca was found in all the groups of queens that were inseminated as at the age of 5 days. Unfortunately as much as 25% of queens inseminated at the age of 5 days did not clear their oviducts and it is expected that they may die within a few days after the treatment. For that reason these queens should not be distributed among the beekeepers.
机译：这项研究是在波兰普劳维蜂业和植物学研究所的蜜蜂育种系完成的。这项工作分别于2006年7月，8月和2007年进行。研究的目的是确定受精后的皇后年龄以及用于授精的精液剂量。确定输卵管的状况和精子的充盈。在皇后5、7或10天时，从自由飞行的高加索无人机中采集了6、8、10 ui精液。在这两年中，对1027位皇后进行了授精和解剖。总共有80.3％的受精皇后已经清除了输卵管，有16.6％的有精液残留，有3.1％的人死亡。与5天龄的输卵管相比，在7天和10天龄授精的女王/王后清除了输卵管（分别为82.3％和86.2％）。在5天大的婴儿中，有23.8％的女王/王后有精液残留。授精后48小时，皇后5、7和10日龄的损失相似（分别为3.8％，3.2％和2.3％）。清理输卵管的女王平均在精囊中拥有319.9万精子。在一组5天大的受精女皇中，发现精子中的精子数量明显增多（平均340万）。在7天和10天龄受精的女王中发现的精子数量较少，而与精液的剂量无关。据称，大多数女王/王后的精子中有300万至450万精子。在5天大的所有受精皇后组中，发现有最高450％的精子中有精子的皇后比例最高。不幸的是，在5天大的年龄中，有25％的女王/王后没有清除其输卵管，预计他们可能会在治疗后几天内死亡。因此，这些女王不应该在养蜂人之间分配。

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