Configuration of membrane-bound proteins by x-ray reflectivity

Chiu-Hao Chen; Sarka Malkova; Wonhwa Cho; Mark L. Schlossman

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Configuration of membrane-bound proteins by x-ray reflectivity

机译：通过X射线反射率构造膜结合蛋白

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In this presentation we review our recent work using x-ray reflectivity to determine the configuration of membrane-bound proteins. The reflectivity data is analyzed in terms of the known crystallo-graphic structure of proteins and a slab model representing the lipid layer to yield an electron density profile of the lipid/protein system. Our recent modified analysis methodology for the lipid/protein system is concisely described in this report. In addition, some results of the configuration of the membrane-bound proteins cPLA_2α-C2, p40~(phox)-PX, and PKCα-C2 are highlighted.

机译：在此演示文稿中，我们回顾了我们最近使用X射线反射率确定膜结合蛋白构型的工作。根据已知的蛋白质晶体结构和代表脂质层的平板模型分析反射率数据，以产生脂质/蛋白质系统的电子密度分布。本报告简要介绍了我们最近对脂质/蛋白质系统的改进分析方法。另外，突出了膜结合蛋白cPLA_2α-C2，p40〜（phox）-PX和PKCα-C2的构型的一些结果。

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来源
《Journal of Applied Physics》 |2011年第10期|p.102215.1-102215.4|共4页
作者
Chiu-Hao Chen; Sarka Malkova; Wonhwa Cho; Mark L. Schlossman;
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作者单位

Department of Physics, University of Illinois at Chicago, Chicago, Illinois 60607, USA;

Department of Physics, University of Illinois at Chicago, Chicago, Illinois 60607, USA;

Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois 60607, USA;

Department of Physics, University of Illinois at Chicago, Chicago, Illinois 60607, USA;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
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1. Configurations of the N-Terminal Amphipathic Domain of the Membrane-Bound M13 Major Coat Protein [J] . Alexander B.Meijer, Ruud.B.Spruijt, Cor J.A.M.Wolfs, Biochemistry . 2001,第16期

机译：膜结合的M13大外壳蛋白的N-末端两性域的构型
2. Lipid Chirality Revisited: A Change in Lipid Configuration Transforms Membrane-Bound Protein Domains [J] . Eghiaian Frederic Biophysical Journal . 2015,第12期

机译：再谈脂质手性：脂质构型的改变改变了膜结合蛋白域。
3. Interfacial Properties and Iron Binding to Bacterial Proteins That Promote the Growth of Magnetite Nanocrystals: X-ray Reflectivity and Surface Spectroscopy Studies [J] . Wenjie Wang, † Wei Bu, † Lijun Wang, Langmuir . 2012,第9期

机译：界面性质和铁与细菌蛋白质的结合，促进磁铁矿纳米晶体的生长：X射线反射率和表面光谱研究
4. Structure and interactions of membrane-bound peptides and proteins studied in detergent micelles by nano-ESI IMS-MS/MS [C] . Frank Sobott, Albert Konijnenberg, Jeroen van Dyck, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2013

机译：纳米IMS-MS / MS在洗涤剂胶束中研究的膜结合肽和蛋白质的结构和相互作用
5. On the use of extended x-ray absorption fine structure spectroscopy to determine the bonding configurations of orthophosphate surface complexes at the goethite/water interface. [D] . Abdala, Dalton Belchior. 2013

机译：在使用扩展的X射线吸收精细结构光谱学来确定针铁矿/水界面处正磷酸盐表面复合物的键合构型。
6. Quantitative Characterization of Configurational Space Sampled by HIV-1 Nucleocapsid Using Solution NMR X-ray Scattering and Protein Engineering [O] . Lalit Deshmukh, Charles D. Schwieters, Alexander Grishaev, -1

机译：使用溶液NMRX射线散射和蛋白质工程技术对HIV-1核壳虫采样的构型空间进行定量表征
7. New and Notable Lipid Chirality Revisited: A Change in Lipid Configuration Transforms Membrane-Bound Protein Domains [O] . Eghiaian, Frederic 2015

机译：再次提出了新的和显着的脂质手性：脂质构型的改变改变了膜结合蛋白结构域。

Configuration of membrane-bound proteins by x-ray reflectivity

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