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Spectrophotomctric Determination of Nitrate in Vegetables Using Phenol

机译:苯酚分光光度法测定蔬菜中的硝酸盐。

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摘要

A rapid and sensitive spectrophotometric method for the determination of nitrate in vegetables is described. The method is based on the measurement of the absorbance of yellow sodium nitrophenoxide formed via the reaction of phenol with the vegetable-based nitrate in presence of sulphuric acid. The analytical concentration of the acid has marked effect on the nitrate determined. The colour development was rapid and remained stable overnight. Analysis of six vegetable samples containing nitrate gave satisfactory mean recoveries of 76 to 123% in 18 determinations. The proposed method is reproducible and sensitive to lower level concentrations.
机译:介绍了一种快速灵敏的分光光度法测定蔬菜中硝酸盐的方法。该方法基于对通过苯酚与植物基硝酸盐在硫酸存在下反应形成的黄色硝基苯甲酸钠的吸光度的测量。酸的分析浓度对测定的硝酸盐有显着影响。显色迅速并且过夜保持稳定。在18次测定中,对六种含硝酸盐的蔬菜样品进行分析,得出令人满意的平均回收率,为76%至123%。所提出的方法是可重现的并且对较低的浓度敏感。

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