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Identification of quantitative trait loci for canine hip dysplasia by two sequential multipoint linkage analyses

机译:两次连续多点连锁分析鉴定犬髋关节发育异常的数量性状位点

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摘要

Canine hip dysplasia (CHD) is characterized by hip laxity and subluxation that can lead to hip osteoarthritis. Studies have shown the involvement of multiple genetic regions in the expression of CHD. Although we have associated some variants in the region of fibrillin 2 with CHD in a subset of dogs, no major disease-associated gene has been identified. The focus of this study is to identify quantitative trait loci (QTL) associated with CHD. Two sequential multipoint linkage analyses based on a reversible jump Markov chain Monte Carlo approach were applied on a cross-breed pedigree of 366 dogs. Hip radiographic trait (Norberg Angle, NA) on both hips of each dog was tested for linkage to 21,455 single nucleotide polymorphisms across 39 chromosomes. Putative QTL for the NA was found on 11 chromosomes (1, 2, 3, 4, 7, 14, 19, 21, 32, 36, and 39). Identification of genes in the QTL region(s) can assist in identification of the aberrant genes and biochemical pathways involving hip dysplasia in both dogs and humans.
机译:犬髋关节发育不良(CHD)的特征是髋关节松弛和半脱位,可导致髋骨关节炎。研究表明,CHD的表达涉及多个遗传区域。尽管我们已经在部分犬中将纤维蛋白2区域的某些变异与CHD相关联,但尚未发现与疾病相关的主要基因。这项研究的重点是确定与冠心病相关的定量性状位点(QTL)。基于可逆跳跃马尔可夫链蒙特卡罗方法的两个顺序多点链接分析应用于366只狗的杂交谱系。测试了每只狗的双髋的髋部放射线特征(Norberg Angle,NA)与39个染色体上的21,455个单核苷酸多态性的连锁性。在11条染色体(1、2、3、4、7、14、19、21、32、36和39)上发现了NA的假定QTL。鉴定QTL区域中的基因可以帮助鉴定犬和人中异常基因和涉及髋关节发育不良的生化途径。

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  • 来源
    《Journal of applied statistics》 |2012年第8期|p.1719-1731|共13页
  • 作者单位

    Department of Statistics, Oklahoma State University, Stillwater, OK 74078, USA;

    Department of Statistics, Oklahoma State University, Stillwater, OK 74078, USA;

    Department of Biostatistics, University at Buffalo - The State University of New York, Buffalo, NY 14214, USA;

    Institute for Genomic Diversity, 175 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA;

    Department of Statistics, Oklahoma State University, Stillwater, OK 74078, USA;

    Department of Statistics, Oklahoma State University, Stillwater, OK 74078, USA;

    Department of Statistics, Oklahoma State University, Stillwater, OK 74078, USA;

    The WALTHAM Centre for Pet Nutrition, Waltham on the Wolds, Leicestershire, UK;

    Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA;

    The WALTHAM Centre for Pet Nutrition, Waltham on the Wolds, Leicestershire, UK;

    Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    reversible jump markov chain monte carlo; multipoint linkage analysis; single nucleotide polymorphism; quantitative trait loci; canine hip dysplasia;

    机译:可逆跳马尔科夫链蒙特卡洛;多点链接分析;单核苷酸多态性数量性状基因座犬髋关节发育不良;

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