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首页> 外文期刊>Journal of biomedical optics >Increased illumination uniformity and reduced photodamage offered by the Lissajous scanning in fiber-optic two-photon endomicroscopy
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Increased illumination uniformity and reduced photodamage offered by the Lissajous scanning in fiber-optic two-photon endomicroscopy

机译:光纤两光子内窥镜中的李沙育扫描可提高照明均匀度并减少光损伤

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摘要

We compare the illumination uniformity and the associated effects of the spiral and Lissajous scanning patterns that are commonly used in an endomicroscope. Theoretical analyses and numerical simulations were first performed to quantitatively investigate the area illumination density in the spiral scanning pattern. The results revealed the potential problem of manifest photodamage due to the very high illumination density in the center of the spiral scan. Similar analyses of the Lissajous scanning pattern, which can be conveniently implemented on the same endomicroscope with no hardware modifications, showed a more uniform illumination density with about an 80-fold reduction in the peak illumination density. To underscore the benefit offered by the improved illumina tion uniformity, we conducted in vitro two-photon fluorescence imaging of cultured cells stained with a LIVE/DEAD viability assay using our home-built, fiber-optic, two-channel endomicroscopy system. Both the spiral and the Lis sajous scans were implemented. Our experimental results showed that cells near the spiral scan center experienced obvious photodamage, whereas cells remained alive over the entire region under the Lissajous beam scanning, confirming the predicted advantage offered by the Lissajous scan over this spiral scan in an endomicroscopy setting.
机译:我们比较了内窥镜中常用的照明均匀性以及螺旋和利萨如扫描模式的相关影响。首先进行理论分析和数值模拟以定量研究螺旋扫描图案中的区域照度密度。结果表明,由于螺旋扫描中心的很高的照明密度,可能存在明显的光损伤问题。可以在同一内窥镜上方便地实施而无需进行硬件修改的李萨如扫描模式的类似分析显示出更均匀的照明密度,峰值照明密度降低了约80倍。为了强调改善的照明均匀性所提供的好处,我们使用自制的光纤两通道内窥镜检查系统,对通过LIVE / DEAD活力测定法染色的培养细胞进行了体外双光子荧光成像。螺旋扫描和Lis锯齿扫描均已实现。我们的实验结果表明,螺旋扫描中心附近的细胞经历了明显的光损伤,而在李沙育光束扫描下,整个区域中的细胞仍然活着,这证实了在内窥镜设置中,李沙育扫描提供的预测优势优于螺旋扫描。

著录项

  • 来源
    《Journal of biomedical optics》 |2012年第2期|p.021108.1-021108.5|共5页
  • 作者单位

    Johns Hopkins University, Department of Biomedical Engineering, Baltimore, Maryland 21205;

    Johns Hopkins University, Department of Biomedical Engineering, Baltimore, Maryland 21205;

    Johns Hopkins University, Department of Biomedical Engineering, Baltimore, Maryland 21205;

    Johns Hopkins University, Department of Biomedical Engineering, Baltimore, Maryland 21205;

    Science and Technology Division, Coming Incorporated, SP-AR-02-2, Corning, New York 14831;

    Johns Hopkins University, Department of Biomedical Engineering, Baltimore, Maryland 21205;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    endomicroscopy; two-photon fluorescence; scanning probe;

    机译:内窥镜检查双光子荧光扫描探针;

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