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Drug quantification in turbid media by fluorescence imaging combined with light-absorption correction using white Monte Carlo simulations

机译:通过荧光成像结合吸光校正(使用白色蒙特卡洛模拟)在混浊介质中进行药物定量

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摘要

Accurate quantification of photosensitizes is in many cases a critical issue in photodynamic therapy. As a noninvasive and sensitive tool, fluorescence imaging has attracted particular interest for quantification in pre-clinical research. However, due to the absorption of excitation and emission light by turbid media, such as biological tissue, the detected fluorescence signal does not have a simple and unique dependence on the fluorophore concentration for different tissues, but depends in a complex way on other parameters as well. For this reason, little has been done on drug quantification in vivo by the fluorescence imaging technique. In this paper we present a novel approach to compensate for the light absorption in homogeneous turbid media both for the excitation and emission light, utilizing time-resolved fluorescence white Monte Carlo simulations combined with the Beer-Lambert law. This method shows that the corrected fluorescence intensity is almost proportional to the absolute fluorophore concentration. The results on controllable tissue phantoms and murine tissues are presented and show good correlations between the evaluated fluorescence intensities after the light-absorption correction and absolute fluorophore concentrations. These results suggest that the technique potentially provides the means to quantify the fluorophore concentration from fluorescence images.
机译:在许多情况下,光敏剂的准确定量是光动力疗法中的关键问题。作为一种非侵入性且灵敏的工具,荧光成像在临床前研究中引起了人们对量化的兴趣。但是,由于混浊介质(例如生物组织)吸收了激发光和发射光,因此检测到的荧光信号对不同组织的荧光团浓度没有简单而唯一的依赖性,而是以复杂的方式取决于其他参数,例如好。因此,通过荧光成像技术对体内药物定量的研究还很少。在本文中,我们提出了一种新颖的方法,可以利用时间分辨荧光白色蒙特卡洛模拟法和比尔-兰伯特定律来补偿均质浑浊介质中对于激发光和发射光的光吸收。该方法表明校正后的荧光强度几乎与绝对荧光团浓度成正比。提出了关于可控组织模型和小鼠组织的结果,并显示了光吸收校正后评估的荧光强度与绝对荧光团浓度之间的良好相关性。这些结果表明该技术潜在地提供了从荧光图像中定量荧光团浓度的手段。

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