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Ethylene Production and Peroxidase Activity in Aphid-Infested Barley

机译:蚜虫侵染的大麦中乙烯的产生和过氧化物酶活性

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The purpose of this work was to investigate whether ethylene is involved in the oxidative and defensive responses of barley to the aphids Schizaphis graminum (biotype C) and Rhopalophum padi. The effect of aphid infestation on ethylene production was measured in two barley cultivars (Frontera and Aramir) that differ in their susceptibility to aphids. Ethylene evolution was higher in plants infested for 16 hr than in plants infested for 4 hr in both cultivars. Under aphid infestation, the production of ethylene was higher in cv. Frontera than in Aramir, the more aphid susceptible cultivar. Ethylene production also increases with the degree of infestation. Maximum ethylene evolution was detected after 16 hr when plants were infested with 10 or more aphids. Comparing the two species of aphids, Schizaphis graminum induced more ethylene evolution than Rhopalosiphum padi. Infestation with S. graminum increased hydrogen peroxide content and total soluble peroxidase activity in cv. Frontera, with a maximum level of H2O2 observed after 20 min of infestation and the maximum in soluble peroxidase activity after 30 min of infestation. When noninfested barley seedlings from cv. Frontera were exposed to ethylene, an increase in hydrogen peroxide and in total peroxidase activity was detected at levels similar to those of infested plants from cv. Frontera. When noninfested plants were treated with 40 ppm of ethylene, the maximum levels of H2O2 and soluble peroxidase activity were at 10 and 40 min, respectively. Ethylene also increased the activity of both cell-wall-bound peroxidases types (ionically and covalently bound), comparable with infestation. These results suggest that ethylene is involved in the oxidative responses of barley plants induced by infestation.
机译:这项工作的目的是调查乙烯是否参与大麦对蚜虫Schizaphis graminum(生物型C)和Rhopalophum padi的氧化和防御反应。在两个大麦品种对蚜虫的敏感性不同的情况下,测量了蚜虫侵染对乙烯产量的影响。在两个品种中,侵染16小时的植物的乙烯释放均高于侵染4小时的植物。在蚜虫侵染下,cv中乙烯的产量较高。额叶比在Aramir中,蚜虫更易感的品种。乙烯的生产也随着侵染程度的增加而增加。当植物受到10或更多种蚜虫侵染后,在16小时后检测到最大的乙烯释放。比较这两种蚜虫,无叶Schizaphis graminum比Rhopalosiphum padi诱导更多的乙烯进化。葡萄球菌的侵染增加了简历中过氧化氢的含量和总可溶性过氧化物酶的活性。感染20分钟后观察到额叶H2 O2 最高,感染30分钟后可溶过氧化物酶活性最高。当不受感染的大麦幼苗从简历。 Frontera暴露于乙烯,检测到过氧化氢和总过氧化物酶活性的升高与来自cv的受侵染植物的水平相似。弗龙特拉。当未受侵染的植物用40 ppm的乙烯处理时,H2O2和可溶性过氧化物酶的最大水平分别为10和40分钟。乙烯还增加了两种细胞壁结合型过氧化物酶的活性(离子和共价结合),与侵染相当。这些结果表明乙烯参与了由大麦引起的大麦植物的氧化反应。

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