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首页> 外文期刊>Journal of Clinical Pathology >CD45RO and CD45RA positive cell populations in idiopathic membranous and IgA glomerulopathy
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CD45RO and CD45RA positive cell populations in idiopathic membranous and IgA glomerulopathy

机译：特发性膜性和IgA肾小球病CD45RO和CD45RA阳性细胞群

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Aims-To immunophenotype and quant-itate glomerular and interstitial inflammatory cells in cases of idiopathic membranous and IgA glomerulopathy; to correlate cell numbers with aspects of clinical data and renal function. Methods-Routine indirect immuno-peroxidase staining was performed on frozen section renal biopsy specimens for T and B lymphocyte related antigens, macrophages and MHC class II antigens. Double immunohistochemical staining was performed to identify CD45RO + and CD45RA+ cells. Results-In IgA glomerulopathy correlations were found relating interstitial cell numbers to creatinine concentration at biopsy (CD45RO+ and CD45RA + cells) and follow up creatinine concentration (CD3 +, CD4 +, CD8 +, CD45RO +, and CD45RA+ cells). Also in IgA glomerulopathy mean arterial pressure at biopsy correlated with interstitial cell numbers and most recent follow up creatinine concentration. There were no correlations between glomerular inflammatory cells and renal function in either disease. Double staining showed that although most glomerular CD45RO+ and CD45RA+ cells were macrophages, positive cells in the interstitium were lymphocytes. The interstitial CD45RO + : RA+ ratio in normal renal biopsy specimens was approximately 5:1; for IgA glomerulopathy it was 1.5 and was 1.0 in idiopathic membranous glomerulopathy. Conclusions-This study demonstrates that interstitial, and not glomerular, inflammatory cell numbers correlate with renal function in primary glomerular disease and that double staining is necessary to interpret positive immunostaining for antigens located on more than one type of inflammatory cell. Detailed investigation of the interstitial CD45RO+ and CD45RA+ cells may give an insight into the pathogenesis of glomerular disease.
机译：目的是针对特发性膜性和IgA肾小球病患者的免疫表型并定量测定肾小球和间质性炎症细胞；使细胞数量与临床数据和肾功能相关。方法：对冰冻切片的肾活检标本进行常规的猪T，B淋巴细胞相关抗原，巨噬细胞和MHC II类抗原常规免疫间接过氧化物酶染色。进行了双重免疫组织化学染色以鉴定CD45RO +和CD45RA +细胞。结果-在IgA肾小球病中，发现间质细胞数目与活检时的肌酐浓度有关（CD45RO +和CD45RA +细胞），并跟进肌酐浓度（CD3 +，CD4 +，CD8 +，CD45RO +和CD45RA +细胞）具有相关性。同样在IgA肾小球病中，活检时的平均动脉压与间质细胞数量和最近的肌酐浓度有关。在这两种疾病中，肾小球炎性细胞与肾功能之间均无相关性。双重染色显示，尽管大多数肾小球CD45RO +和CD45RA +细胞是巨噬细胞，但间质中的阳性细胞却是淋巴细胞。正常肾活检标本中的间质CD45RO +：RA +比约为5：1；对于IgA肾小球病，特发性膜性肾小球病为1.5​​，而1.0为1.0。结论-这项研究表明，在原发性肾小球疾病中，肾小球间质而非肾小球炎性细胞数量与肾功能相关，并且双重染色对于解释位于一种以上炎性细胞上的抗原的阳性免疫染色是必需的。对间质CD45RO +和CD45RA +细胞的详细研究可能有助于了解肾小球疾病的发病机理。

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