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首页> 外文期刊>Journal of Crystal Growth >Effects of temperature, pressure, and pH on the solubility of triclinic lysozyme crystals
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Effects of temperature, pressure, and pH on the solubility of triclinic lysozyme crystals

机译：温度，压力和pH值对三斜溶菌酶晶体溶解度的影响

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摘要

We measured the temperature dependence of the solubility, C_e, of triclinic lysozyme crystals on temperature at two pressures (0.1 and 100 MPa) and five pHs (4.3, 4.4, 4.5, 4.6, and 4.7) by in situ observation of the morphological changes in the crystals. The solubility increased with increase in temperature and pressure. The enthalpies of dissolution (AH) decreased with increase in pressure when we used the same 50 mM sodium acetate buffer solution (pH=4.5 at 0.1 MPa). The effects of pH on solubility were somehow complicated. Although the solubilities at pH 4.7 did not differ from those at pH 4.5, those at pH 4.3 were significantly larger than those at pH 4.5. Since the pH of an acetate buffer is known to decrease from 4.71 to 4.52 at 98.1 MPa and 25℃ [22], the pH of the acetate buffer (pH 4.5 at 0.1 MPa) decreased to pH～4.3 as pressure increased to 100 MPa. Thus, from the viewpoint of "constant pH", AH increased with increase in pressure, since AH at pH 4.3 and 0.1 MPa was smaller than that at 100 MPa. This inconsistency is probably due to the change in the hydration state of the crystal and solution with decreasing pH (from 4.5 to 4.3).
机译：我们通过在原位观察三叉戟溶菌酶晶体的形态变化来测量其在两个压力（0.1和100 MPa）和五个pH（4.3、4.4、4.5、4.6和4.7）下的溶解度C_e对温度的温度依赖性。晶体。溶解度随着温度和压力的增加而增加。当我们使用相同的50 mM醋酸钠缓冲溶液（0.1 MPa，pH = 4.5）时，溶解焓（AH）随压力增加而降低。 pH对溶解度的影响有些复杂。尽管pH 4.7的溶解度与pH 4.5的溶解度没有区别，但pH 4.3的溶解度明显大于pH 4.5的溶解度。由于已知乙酸盐缓冲液的pH在98.1 MPa和25℃时从4.71降至4.52 [22]，因此随着压力增加至100 MPa，乙酸盐缓冲液的pH（0.1 MPa下的pH 4.5）降低至pH〜4.3。因此，从“恒定pH”的观点出发，pH为4.3、0.1MPa时的AH比100MPa时的AH小，因此AH随着压力的增加而增加。这种不一致可能是由于晶体和溶液的水合状态随pH值降低（从4.5降低至4.3）而变化。

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  • 来源
    《Journal of Crystal Growth》 |2011年第1期|p.1085-1088|共4页
  • 作者

    Yoshihisa Suzuki; Emi Konda; Hironori Hondoh; Katsuhiro Tamura;

  • 作者单位

    Department of Life System, Institute of Technology and Science, The University of Tokushima, 2-1 Minamijosanjima, Tokushima 770-8506, Japan;

    Tomita Pharmaceutical Co., Ltd., Naruto, Tokushima 771-0360, Japan;

    Memuro Upland Farming Research Station, National Agricultural Research Center for Hokkaido Region, 9-4 Shinsei Minami, Memuro-cho, Kasai-gun, Hokkaido 082-0081, Japan;

    Department of Life System, Institute of Technology and Science, The University of Tokushima, 2-1 Minamijosanjima, Tokushima 770-8506, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    A1. Biocrystallization; A1. Phase diagrams; A1. Phase equilibria; A1. Solubility; B1. Lysozyme;

    机译：A1。生物结晶;A1。相图;A1。相平衡;A1。溶解度B1。溶菌酶;

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