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首页> 外文期刊>Journal of Crystal Growth >Estimated effects of silicone glue on protein crystal growth
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Estimated effects of silicone glue on protein crystal growth

机译：估计硅酮胶对蛋白质晶体生长的影响

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摘要

Silicone glue (modified silicone polymer) is widely used for both experiments involving inorganic crystal growth and those involving organic materials like proteins. This material is very useful for building a hand-made experiment setup or for fixing protein crystals to specific locations. Though silicone glue is regarded as harmful to proteins, no systematic verification was performed to investigate its impurity effects on protein crystal growth. We focused on and estimated the impurity effects of silicone glue on protein crystal growth.rnHen egg white lysozyme (HEWL) was used as a model protein. Surface morphology and step velocity of tetragonal lysozyme crystals in the presence and absence of silicone glue were investigated by laser confocal interference contrast microscopy (LCM-DIM). The surface morphology of a tetragonal lysozyme crystal in the presence of silicone glue corresponded to that grown in a lysozyme solution without silicone glue. The dependency of step velocities on supersaturation in the presence of silicone glue also exhibited the same tendency as that of a glue-free system. These two phenomena indicate that the silicone glue did not act as an impurity on lysozyme crystals. Therefore, we conclude that silicone glue is an effective material for various unique experiments involving protein crystals or for applying new methods to create large, high-quality protein crystals.
机译：硅酮胶（改性硅酮聚合物）被广泛用于涉及无机晶体生长的实验和涉及有机材料（如蛋白质）的实验。这种材料对于建立手工实验装置或将蛋白质晶体固定到特定位置非常有用。尽管有机硅胶被认为对蛋白质有害，但并未进行系统验证以研究其杂质对蛋白质晶体生长的影响。我们重点研究并估算了硅酮胶对蛋白质晶体生长的杂质影响。rn鸡蛋清溶菌酶（HEWL）被用作模型蛋白质。通过激光共聚焦干涉对比显微镜（LCM-DIM）研究了在存在和不存在有机硅胶的情况下四方溶菌酶晶体的表面形态和阶跃速度。在硅酮胶存在下，四方溶菌酶晶体的表面形态与在没有硅酮胶的溶菌酶溶液中生长的形态相对应。在有机硅胶存在下，步速对过饱和度的依赖性也表现出与无胶体系相同的趋势。这两种现象表明硅酮胶在溶菌酶晶体上没有作为杂质。因此，我们得出结论，硅酮胶是一种有效的材料，可用于涉及蛋白质晶体的各种独特实验或用于应用新方法来创建大型，高质量蛋白质晶体的材料。

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  • 来源
    《Journal of Crystal Growth》 |2010年第19期|p.2771-2774|共4页
  • 作者

    Mihoko Maruyama; rnNoriko Shimizu; rnShigeru Sugiyama; rnYoshinori Takahashi; rnHiroaki Adachi; rnKazufumi Takano; rnSatoshi Murakami; rnTsuyoshi Inoue; rnHiroyoshi Matsumura; rnYusuke Mori;

  • 作者单位

    Graduate School of Engineering, Osaka University, 2-1, Yamadaoka, Suita, Osaka 565-0871, Japan CREST JST, Suita, Osaka 565-0871, Japan;

    rnGraduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan CREST JST, Suita, Osaka 565-0871, Japan;

    rnGraduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan CREST JST, Suita, Osaka 565-0871, Japan;

    rnGraduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan CREST JST, Suita, Osaka 565-0871, Japan;

    rnGraduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan SOSHO Inc., Osaka 541-0053, Japan CREST JST, Suita, Osaka 565-0871, Japan;

    rnGraduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan SOSHO Inc., Osaka 541-0053, Japan CREST JST, Suita, Osaka 565-0871, Japan;

    rnSOSHO Inc., Osaka 541-0053, Japan Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan CREST JST, Suita, Osaka 565-0871, Japan;

    rnGraduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan SOSHO Inc., Osaka 541-0053, Japan CREST JST, Suita, Osaka 565-0871, Japan;

    rnGraduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan SOSHO Inc., Osaka 541-0053, Japan CREST JST, Suita, Osaka 565-0871, Japan;

    rnGraduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan SOSHO Inc., Osaka 541-0053, Japan CREST JST, Suita, Osaka 565-0871, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    A1. Impurities; A1. Optical microscopy; A1. Surface processes; A2. Growth from solutions; B1. Lysozyme; B1. Protein;

    机译：A1。杂质;A1。光学显微镜A1。表面工艺;A2。解决方案的增长;B1。溶菌酶;B1。蛋白;

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