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Adaptation of in-situ microscopy for crystallization processes

机译：原位显微镜适应结晶过程

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摘要

In biotechnological and pharmaceutical engineering, the study of crystallization processes gains importance. An efficient analytical inline sensor could help to improve the knowledge about these processes in order to increase efficiency and yields. The in-situ microscope (ISM) is an optical sensor developed for the monitoring of bioprocesses. A new application for this sensor is the monitoring in downstream processes, e.g. the crystallization of proteins and other organic compounds.rnThis contribution shows new aspects of using in-situ microscopy to monitor crystallization processes. Crystals of different chemical compounds were precipitated from supersaturated solutions and the crystal growth was monitored. Exemplified morphological properties and different forms of crystals could be distinguished on the basis of offline experiments. For inline monitoring of crystallization processes, a special 0.5 L stirred tank reactor was developed and equipped with the in-situ microscope. This reactor was utilized to carry out batch experiments for crystallizations of O-acetylsalicyclic acid (ASS) and hen egg white lysozyme (HEWL). During the whole crystallization process, the in-situ microscope system acquired images directly from the crystallization broth. For the data evaluation, an image analysis algorithm was developed and implemented in the microscope analysis software.
机译：在生物技术和制药工程中，结晶过程的研究变得越来越重要。高效的在线分析传感器可以帮助提高有关这些过程的知识，从而提高效率和产量。原位显微镜（ISM）是一种用于监视生物过程的光学传感器。该传感器的新应用是下游过程的监控，例如蛋白质和其他有机化合物的结晶。这项贡献显示了使用原位显微镜监控结晶过程的新方面。从过饱和溶液中沉淀出不同化合物的晶体，并监测晶体的生长。可以根据离线实验来区分示例性的形态学特性和不同形式的晶体。为了在线监测结晶过程，开发了特殊的0.5 L搅拌釜反应器，并配备了原位显微镜。该反应器用于进行O-乙酰水杨酸（ASS）和鸡蛋清溶菌酶（HEWL）的结晶的分批实验。在整个结晶过程中，原位显微镜系统直接从结晶肉汤中获取图像。为了进行数据评估，开发了图像分析算法，并在显微镜分析软件中实现了该算法。

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  • 来源
    《Journal of Crystal Growth》 |2009年第17期|4193-4198|共6页
  • 作者

    A. Bluma; T. Hoepfner; G. Rudolph; P. Lindner; S. Beutel; B. Hitzmann; T. Scheper;

  • 作者单位

    Institut fuer Technische Chemie, Gottfried Wilhelm Leibniz Universitaet Hannover, Callinstrasse 5, 30167 Hannover, Germany;

    Institut fuer Technische Chemie, Gottfried Wilhelm Leibniz Universitaet Hannover, Callinstrasse 5, 30167 Hannover, Germany;

    Institut fuer Technische Chemie, Gottfried Wilhelm Leibniz Universitaet Hannover, Callinstrasse 5, 30167 Hannover, Germany;

    Institut fuer Technische Chemie, Gottfried Wilhelm Leibniz Universitaet Hannover, Callinstrasse 5, 30167 Hannover, Germany;

    Institut fuer Technische Chemie, Gottfried Wilhelm Leibniz Universitaet Hannover, Callinstrasse 5, 30167 Hannover, Germany;

    Institut fuer Technische Chemie, Gottfried Wilhelm Leibniz Universitaet Hannover, Callinstrasse 5, 30167 Hannover, Germany;

    Institut fuer Technische Chemie, Gottfried Wilhelm Leibniz Universitaet Hannover, Callinstrasse 5, 30167 Hannover, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    A1. Biocrystallization; A2. Crystal growth; A2. Crystal morphology; B1. ASS; B1. Lysozyme; B3. In-situ microscopy;

    机译：A1。生物结晶;A2。晶体生长;A2。晶体形态B1。屁股;B1。溶菌酶;B3。原位显微镜;

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