...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Crystal Growth >Phase transitions and precrystallization processes in a water-protein-electrolyte system
【24h】

Phase transitions and precrystallization processes in a water-protein-electrolyte system

机译：水-蛋白质-电解质系统中的相变和预结晶过程

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To study the conditions under which supramolecular dynamic aggregates of proteins (clusters) are formed in protein solutions, the thermodynamic stability of the model system water-biopolymer-electrolyte was analyzed. The analytical expression of the coefficient of the stability of the system (partial deriv_μ1/partial deriv m2)_(m3) was obtained, where μ_1 is a chemical potential of water and m_2 and m_3 are molar concentrations of the biopolymer and the electrolyte. The expression was used to obtain equations of spinodal and two quasi-spinodals that converge at one point in phase diagram. They correspond to the lines of critical and supercritical phase transitions (PTs) that give rise to the various structural-dynamic states of solution. These equations relate the critical composition of the system (m_2m_3)_(cr) to the specific characteristics of the biopolymer and electrolyte (m_2m_3)_(cr) = f(v, z, Δ), where v is the number of the electrolyte ions that interact with the biopolymer, z is its charge, and Δ is the function of concentration-dependent variation in the coefficient of activity of the electrolyte. In the region of one of the quasi-spinodals, in a narrow electrolyte concentration range, the most favorable conditions for the formation of crystalline-phase nuclei have been created. The author presents experimental data obtained using the EPR spin-label method that provide evidence for PTs in solutions of serum albumin molecules which are in a qualitative agreements with phase diagram predictions. The mechanism of protein cluster formation is discussed.
机译：为了研究在蛋白质溶液中形成蛋白质（簇）的超分子动态聚集体的条件，分析了模型系统水-生物聚合物-电解质的热力学稳定性。得到系统稳定性系数的解析表达式（偏导数μ1/偏导数m2）_（m3），其中μ_1是水的化学势，m_2和m_3是生物聚合物和电解质的摩尔浓度。该表达式用于获得在相图中某一点收敛的旋节线和两个准螺线管的方程。它们对应于临界和超临界相变（PT）的线，这些线引起溶液的各种结构动力学状态。这些方程将系统的关键组成（m_2m_3）_（cr）与生物聚合物和电解质的特定特征相关（m_2m_3）_（cr）= f（v，z，Δ），其中v是电解质的数量与生物聚合物相互作用的离子，z是其电荷，Δ是电解质活性系数的浓度依赖性变化的函数。在准尖晶石之一的区域中，在较窄的电解质浓度范围内，已经形成了形成晶相核的最有利条件。作者介绍了使用EPR自旋标记法获得的实验数据，这些数据为与相图预测定性吻合的血清白蛋白分子溶液中的PT提供了证据。讨论了蛋白质簇形成的机理。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号