Membrane crystallization of lysozyme: kinetic aspects

Gianluca Di Profio; Efrem Curcio; Alberto Cassetta; Doriano Lamba; Enrico Drioli

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Membrane crystallization of lysozyme: kinetic aspects

机译：溶菌酶的膜结晶：动力学方面

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The relevant kinetic aspects related to an innovative method of biological macromolecules crystallization based on microporous hydrophobic membranes, used both as active surfaces to promote heterogeneous nucleation and as a mass-transfer apparatus to concentrate macromolecular solutions by solvent removal in vapour phase, have been evaluated. Polypropylene membranes, supplied in the form of hollow fibres, have been aligned in a versatile system, designed for an on-line spectrophotometric monitoring of hen egg white lysozyme crystallizing solutions (experimental conditions: 0.1 M NaAc/HAc Buffer pH 4.6, 0.5-5.8% wt/vol NaCl, 20℃). The turbidity measurements have been exploited in order to follow: (ⅰ) the induction time of crystallization, (ⅱ) the early stage nucleation kinetics based on the Rayleigh scattering theory, and (ⅲ) the crystal growth rate (coupled with data evaluated from image-analysis carried out by optical microscopy) under a model hypothesis of exponential growth of clusters. The crystals have been qualitatively assessed by an X-ray crystallographic analysis carried out at the synchrotron light laboratory ELETTRA.

机译：已经评估了与基于微孔疏水膜的生物大分子结晶创新方法有关的相关动力学方面，该方法既用作活性表面以促进非均相成核，又用作传质装置以通过汽相去除溶剂来浓缩大分子溶液。以中空纤维形式提供的聚丙烯膜已在通用系统中对齐，设计用于在线分光光度法监测鸡蛋清溶菌酶结晶溶液（实验条件：0.1 M NaAc / HAc缓冲液pH 4.6，0.5-5.8 ％wt / vol NaCl，20℃）。为了进行浊度测量，我们进行了如下研究：（ⅰ）结晶诱导时间，（ⅱ）基于瑞利散射理论的早期成核动力学，以及（ⅲ）晶体生长速率（结合从图像中评估的数据） -光学显微镜进行的分析）是在簇呈指数增长的模型假设下进行的。通过在同步加速器光实验室ELETTRA上进行的X射线晶体学分析定性评估了晶体。

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来源
《Journal of Crystal Growth》 |2003年第4期|p.359-369|共11页
作者
Gianluca Di Profio; Efrem Curcio; Alberto Cassetta; Doriano Lamba; Enrico Drioli;
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作者单位

Research Institute on Membrane Technology, ITM-CNR, do University of Calabria, Via P. Bucci Cuho 17/C, I-87030 Arcavacata di Rende, CS, Italy;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
原文格式 PDF
正文语种 eng
中图分类晶体学;
关键词
A1. heterogeneous nucleation; A1. microporous hydrophobic membranes; A1. protein crystallization; A1. X-ray diffraction; A3. membrane crystallization; B1. lysozyme;

机译：A1。异相成核;A1。微孔疏水膜;A1。蛋白质结晶;A1。 X射线衍射;A3。膜结晶;B1。溶菌酶;

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专利

1. Toward Further Understanding of Lysozyme Crystallization: Phase Diagram, Protein-Protein Interaction, Nucleation Kinetics, and Growth Kinetics [J] . Liu YX, Wang XJ, Ching CB Crystal growth & design . 2010,第2期

机译：进一步理解溶菌酶的结晶：相图，蛋白质-蛋白质相互作用，成核动力学和生长动力学
2. Kinetics and equilibria of lysozyme precipitation and crystallization in concentrated ammonium sulfate solutions [J] . Cheng YC, Lobo RF, Sandler SI, Biotechnology and Bioengineering . 2006,第1期

机译：浓硫酸铵溶液中溶菌酶沉淀和结晶的动力学和平衡
3. Comparison of the crystallization kinetics of canavalin and lysozyme [J] . Caraballo KG, Baird JK, Ng JD Crystal growth & design . 2006,第4期

机译：蚕豆素和溶菌酶的结晶动力学比较
4. Salt crystal morphology modifid and solution recovery improved membrane crystallization:kinetic and hybrid control mechanism [C] . Xiaobin Jiang, Guannan Li, Dapeng Lu, International Forum on Crystal Engineering and Smart Manufacturing;Tianjin University;Tianjin Science and Technology Association . -1

机译：盐晶素形态改性和溶液回收改善膜结晶：动力学和杂交控制机制
5. Effect of pressure on the kinetics of lysozyme crystallization [D] . Saikumar, Mungara Venkata. 1996

机译：压力对溶菌酶结晶动力学的影响
6. Monomer concentrations and dimerization constants in crystallizing lysozyme solutions by dialysis kinetics. [O] . L J Wilson, L Adcock-Downey, M L Pusey 1996

机译：通过透析动力学结晶溶菌酶溶液中的单体浓度和二聚常数。
7. Study on Nucleation Kinetics of Lysozyme Crystallization [O] . Lin C, Zhang Y, Liu JJ, 2017

机译：溶菌酶结晶的成核动力学研究

Membrane crystallization of lysozyme: kinetic aspects

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