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首页> 外文期刊>Journal of dairy research >Identification and verification of differentially expressed genes in yak mammary tissue during the lactation cycle
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Identification and verification of differentially expressed genes in yak mammary tissue during the lactation cycle

机译：泌乳循环期间牦牛乳腺组织差异表达基因的鉴定及验证

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摘要

Yaks (Bos grunniens) live primarily in the Qinghai-Tibetan plateau (altitude: 2000-5000 m). Their milk presents unusual characteristics, containing large amounts of solids including fat and protein, and it is, therefore, important to understand the genetic makeup of the yak. To identify potentially critical genes playing a role in yak mammary tissue from colostrum to mature milk phase of lactogenesis, the early lactation (colostrum) stage (ELS; day 1 after parturition) and mature lactation (milk) stage (MLS; day 15) were chosen for comparison. An ELS-specific cDNA library was established by suppression subtractive hybridization and 25 expressed sequence tags at ELS were identified by sequencing and alignment. To further confirm our results the expression levels of 21 genes during the lactation cycle were measured using quantitative real-time RT-PCR (qRT-PCR). The qRT-PCR results confirmed 9 significantly up-regulated genes at ELS vs. MLS in yak mammary tissue, in which the L-amino acid oxidase 1 (LAO1) and collagen, type Ⅰ, alpha Ⅰ (COL1A1) were the most significantly up-regulated. During the lactation cycle, the highest expression of some milk fat genes (i.e., XDH and FABP3) in yak mammary tissue appears earlier than that in dairy cow. Our data also indicate MYC potentially playing a central role through putative regulation of COL1A1, CD44, SPARC, FASN and GPAM.
机译：Yaks（Bos Grunniens）主要生活在青海高原（海拔：2000-5000米）。他们的牛奶呈现出不寻常的特征，含有大量的固体，包括脂肪和蛋白质，因此，了解牦牛的遗传构成很重要。鉴定潜在的临界基因在从初乳中从亚克斯乳腺组织中发挥作用的临界基因，即泌乳生成的成熟牛奶阶段，早期哺乳期（初乳）阶段（ELS;第1天后第1天）和成熟的哺乳期（牛奶）阶段（MLS;第15天）是选择进行比较。通过抑制消减生成杂交和ELS的25个表达序列标记通过测序和比定来确定ELS特异性cDNA文库。为了进一步证实我们的结果，使用定量实时RT-PCR（QRT-PCR）测量哺乳期循环期间21基因的表达水平。 QRT-PCR结果证实了牦牛乳腺组织中ELS与MLS的显着上调基因，其中L-氨基酸氧化酶1（LAO1）和胶原蛋白，Ⅰ型，αⅠ（COL1A1）是最显着的 - 解释。在哺乳期循环期间，在牦牛乳腺组织中一些乳脂基因（即XDH和FABP3）的最高表达早于奶牛的早期表达。我们的数据还指出Myc通过调整Col1A1，CD44，SPARC，FASN和GPAM的规定潜在地扮演核心作用。

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  • 来源
    《Journal of dairy research》 |2020年第2期|158-165|共8页
  • 作者

    Mao Yuan; Wei Xia; Xiaolei Zhang; Yongtao Liu; Mingfeng Jiang;

  • 作者单位

    The Research Institute of Qinghai-Tibet Plateau Southwest Minzu University Chengdu China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Conservation and Exploitation State Ethnic Affairs Commission and Ministry of Education Chengdu China;

    The Research Institute of Qinghai-Tibet Plateau Southwest Minzu University Chengdu China College of Life Science and Technology Southwest Minzu University Chengdu China;

    College of Life Science and Technology Southwest Minzu University Chengdu China;

    College of Life Science and Technology Southwest Minzu University Chengdu China;

    The Research Institute of Qinghai-Tibet Plateau Southwest Minzu University Chengdu China College of Life Science and Technology Southwest Minzu University Chengdu China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Conservation and Exploitation State Ethnic Affairs Commission and Ministry of Education Chengdu China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Differentially expressed genes; early lactation stage; mammary tissue; Yak;

    机译：差异表达基因;早期哺乳期;乳房组织;牦牛;

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