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首页> 外文期刊>Journal of dairy research >Feeding level regulates the expression of some genes involved with programed cell death and remodeling in goat and sheep mammary tissue
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Feeding level regulates the expression of some genes involved with programed cell death and remodeling in goat and sheep mammary tissue

机译：喂养水平调节一些基因的表达，参与编程的细胞死亡和在山羊和绵羊乳腺组织中的重塑

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摘要

Mammary tissue (MT) turnover is characterized by programed cell death and remodeling which might be affected by both feeding level and animal species. Thus, twenty-four dairy goats and the same number of sheep were assigned to three homogenous sub-groups per animal species and fed the same diet in quantities which met 70% (FL70), 100% (FL100) and 130% (FL130) of their daily energy and crude protein requirements. Individual MT samples were taken by biopsy from the animals on the 30th and 60th experimental day. The results showed, in the first sampling time, a significant reduction in the mRNA abundance for selected genes involved in programed cell death in both FL 70 fed goats (STAT3 and BECN1) and sheep (CASPASE8 and BECN1) compared with the respective FL100 groups. The FL130, in comparison with the FL100, caused a significant increase in transcripts accumulation of STAT3 gene in both sampling times and CASPASE8 gene in the second sampling time in goat MT, while the opposite happened for the mRNA expression of CASPASE8 and BECN1 genes in sheep MT, but only in the first sampling time. Moreover, a significant up regulation in the mRNA levels of MMP2 gene in MT of FL130 fed sheep was observed. The FL130, in comparison with the FL70, caused an enhancement in the mRNA expression levels of BECNl, CASPASE8, BAX and STAT3 genes in goat MT only. It was also shown that apoptosis and autophagy can be affected simultaneously by the feeding level. Overfeeding affects MT programed cell death and remodeling by a completely different way in goats than sheep. In conclusion, feeding level and animal species have strong effects on both MT programed cell death (apoptosis and autophagy) and remodeling but the molecular mechanisms need further investigation.
机译：乳腺组织（MT）营业额的特征是通过喂养水平和动物物种影响的编程细胞死亡和重塑。因此，二十四只乳制品山羊和相同数量的绵羊每只动物物种分配到三个均质亚组，并喂食相同的饮食，达到70％（FL70），100％（FL100）和130％（FL130）他们的日常能量和粗蛋白质要求。在第30和第60次实验日，通过动物的活组织检查拍摄单个MT样品。结果表明，在第一取样时间中，与各自的FL100基团相比，在FL 70喂养山羊（STAT3和BEDN1）和羊（Caspase8和Becn1）中，参与所选基因的MRNA丰富的MRNA丰富的显着降低。与FL100相比，FL130导致在山羊MT中的第二个采样时间中的取样时间和Caspase8基因中的成绩单累积的显着增加，而Caspase8和羊在羊的MRNA表达中发生相反MT，但只在第一次采样时间。此外，观察到FL130饲料MT中MMP2基因MRNA水平的显着调控。与FL70相比，FL130在山羊MT中仅引起了BECNL，CASPASE8，BAX和Stat3基因的mRNA表达水平。还显示出细胞凋亡和自噬可以通过饲养水平同时影响。过度喂养会影响MT编程的细胞死亡，并在山羊中的完全不同的方式改造而不是羊。总之，喂养水平和动物物种对MT编程细胞死亡（细胞凋亡和自噬）和重塑具有很强的影响，但分子机制需要进一步调查。

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  • 来源
    《Journal of dairy research》 |2020年第4期|448-455|共8页
  • 作者

    Eleni Tsiplakou; Christina Mitsiopoulou; Dimitrios Skliros; Alexandras Mavrommatis; Emmanouil Flemetakis;

  • 作者单位

    Department of Animal Science School of Animal Biosciences Agricultural University of Athens Iera Odos 75 GR-11855 Athens Greece;

    Department of Animal Science School of Animal Biosciences Agricultural University of Athens Iera Odos 75 GR-11855 Athens Greece;

    Laboratory of Molecular Biology Department of Biotechnology School of Applied Biology and Biotechnology Agricultural University of Athens Iera Odos 75 GR-11855 Athens Greece;

    Department of Animal Science School of Animal Biosciences Agricultural University of Athens Iera Odos 75 GR-11855 Athens Greece;

    Laboratory of Molecular Biology Department of Biotechnology School of Applied Biology and Biotechnology Agricultural University of Athens Iera Odos 75 GR-11855 Athens Greece;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Apoptosis; autophagy; feeding level; genes;

    机译：细胞凋亡;自噬;喂养水平;基因;

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