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首页> 外文期刊>Journal of dairy research >Factors influencing milk osteopontin concentration based on measurements from Danish Holstein cows
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Factors influencing milk osteopontin concentration based on measurements from Danish Holstein cows

机译：影响牛奶骨桥蛋白浓度的因素基于来自丹麦荷斯坦奶牛的测量

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摘要

Our objective was to determine the content of the bioactive protein osteopontin (OPN) in bovine milk and identify factors influencing its concentration. OPN is expressed in many tissues and body fluids, with by far the highest concentrations in milk. OPN plays a role in immunological and developmental processes and it has been associated with several milk pro-duction traits and lactation persistency in cows. In the present study, we report the develop-ment of an enzyme linked immunosorbent assay (ELISA) for measurement of OPN in bovine milk. The method was used to determine the concentration of OPN in milk from 661 individual Danish Holstein cows. The median OPN level was determined to 21.9 mg/1 with a pronounced level of individual variation ranging from 0.4 mg/1 to 67.8 mg/1. Breeding for increased OPN in cow's milk is of significant interest, however, the heritability of OPN in milk was found to be relatively low, with an estimated value of 0.19 in the current dataset. The variation explained by the herd was also found to be low suggesting that OPN levels are not affected by farm management or feeding. Interestingly, the concentration of OPN was found to increase with days in milk and to decrease with parity.
机译：我们的目的是确定牛奶中生物活性蛋白骨桥蛋白（OPN）的含量，并确定影响其浓度的因素。 OPN在许多组织和体液中表达，迄今为止牛奶中的最高浓度。 OPN在免疫和发育过程中发挥作用，它与奶牛的几种牛奶生产性状和哺乳期持久性有关。在本研究中，我们报告了酶联免疫吸附试验（ELISA）的发育，用于测量牛奶中的OPN。该方法用于从661个个体丹麦荷斯坦奶牛中确定牛奶中OPN浓度。中值OPN水平测定为21.9mg / 1，个体变化水平范围为0.4mg / 1至67.8 mg / 1。母牛牛奶中o​​pn增加的育种具有重要兴趣，然而，发现OPN在牛奶中的可遗传性相对较低，估计值为0.19的数据集。群体解释的变异也被发现很低，表明OPN水平不受农场管理或喂养的影响。有趣的是，发现OPN的浓度随着牛奶的日子而增加，并随着平价减少。

著录项

  • 来源
    《Journal of dairy research》 |2021年第1期|89-94|共6页
  • 作者

    Brian Christensen; Elias D. Zachariae; Nina A. Poulsen; Albert J. Buitenhuis; Lotte B. Larsen; Esben S. Sorensen;

  • 作者单位

    Department of Molecular Biology and Genetics Science Park Aarhus University Aarhus Denmark CiFOOD Aarhus University Centre for Innovative Food Research Aarhus Denmark;

    Department of Molecular Biology and Genetics Science Park Aarhus University Aarhus Denmark;

    CiFOOD Aarhus University Centre for Innovative Food Research Aarhus Denmark Department of Food Science Aarhus University Aarhus Denmark;

    CiFOOD Aarhus University Centre for Innovative Food Research Aarhus Denmark Center for Quantitative Genetics and Genomics Aarhus University Tjele Denmark;

    CiFOOD Aarhus University Centre for Innovative Food Research Aarhus Denmark Department of Food Science Aarhus University Aarhus Denmark;

    Department of Molecular Biology and Genetics Science Park Aarhus University Aarhus Denmark CiFOOD Aarhus University Centre for Innovative Food Research Aarhus Denmark;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Bioactive milk protein; ELISA; heritability; Osteopontin;

    机译：生物活性牛奶蛋白;ELISA;遗传性;骨桥素;

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