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Proteomic profiling of ovine milk after grading up

机译：渐变后绵羊牛奶蛋白质组学分析

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摘要

We have previously bred Chinese local dairy sheep through grading up with local Small-Tailed Han (STH) sheep as female parent and DairyMeade (DM) sheep as male parent. In this research communication we characterize the whey protein profile of STH sheep and their offspring (F1, F2) to reveal physiological differences and variation in milk traits. A total of 1032 whey proteins were identified through tandem mass tag labeling (TMT) proteome profiling. Three proteins were significantly differentially abundant between Fl and STH milk, six between F2 and STH milk and five between Fl and F2 milk. In terms of differential changes between generations, WASHC4 and CUTA of Fl and Ig-like domain-containing protein of F2 milk were dominant whey proteins. Overall, the results showed that the whey protein profiles of different generations varied little. The crossbreeds of STH and DM sheep would be suitable for the development of the Chinese local sheep milk industry, and the F2 may be a better population for sheep milk production.
机译：我们之前曾在用当地小尾汉（sth）羊作为女性父母和达布里大（dm）羊作为男性父母进行渐变，我们养成了中国本地奶牛。在这项研究中，我们将STH羊的乳清蛋白曲线及其后代（F1，F2）表征为揭示牛奶特征的生理差异和变化。通过串联质量标记（TMT）蛋白质组分析，鉴定了总共1032个乳清蛋白。在F1和STH牛奶之间有三种蛋白质在F2和STH牛奶之间具有显着差异丰富，六种牛奶和F1和F2牛奶之间的五个。就几代人的差异变化而言，F2牛奶的F1和IG样结构域的蛋白质之间的差异变化是显性乳清蛋白。总的来说，结果表明，不同一代的乳清蛋白谱不同。 STH和DM羊的杂交将适合于中国局部绵羊奶业的发展，F2可能是羊奶生产的更好的人口。

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  • 来源
    《Journal of dairy research》 |2021年第2期|201-204|共4页
  • 作者

    Xiaohu Su; Zhong Zheng; Liguo Zhang; Urhan Bai; Ying Ma; Yingjie Dou; Xiaoran Zhang; Guanghua Su; Ningcong Zhou; Guangpeng Li; Li Zhang;

  • 作者单位

    The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China College of Life Science Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China;

    The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China College of Life Science Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China;

    Ulanqab Animal Husbandry Workstation Ulanqab Agriculture and Animal Husbandry Bureau Ulanqab Inner Mongolia Autonomous Region 012000 PR China;

    The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China College of Life Science Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China;

    The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China College of Life Science Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China;

    The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China College of Life Science Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China;

    The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China College of Life Science Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China;

    The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China College of Life Science Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China;

    Ulanqab Animal Husbandry Workstation Ulanqab Agriculture and Animal Husbandry Bureau Ulanqab Inner Mongolia Autonomous Region 012000 PR China;

    The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China College of Life Science Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China;

    The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China College of Life Science Inner Mongolia University Hohhot Inner Mongolia Autonomous Region 010018 PR China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Milk; ovine; proteomic;

    机译：牛奶;绵羊;蛋白质组学;

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