• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of dairy research >Identification of bovine mastitis pathogens using MALDI-TOF mass spectrometry in Brazil
【24h】

Identification of bovine mastitis pathogens using MALDI-TOF mass spectrometry in Brazil

机译：巴西马尔迪-TOF质谱法鉴定牛乳腺炎病原体

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this Research Communication we evaluate the use of matrix-assisted laser desorption/ion-ization time-of-flight mass spectrometry (MALDI-TOF MS) to identify 380 bacteria isolated from cases of bovine mastitis in Brazil. MALDI-TOF MS identifications were compared to previous identifications by biochemical tests and 16S rRNA sequencing. MALDI-TOF MS achieved a typeability of 95.5%. The accuracy of MALDI-TOF MS for the identification of Staphylococcus isolates was 93.2%. The agreement between MALDI-TOF MS and biochemical identification of Streptococcus agalactiae was 96%, however, the agreement between these techniques for identifying other catalase-negative, Gram-positive cocci was lower. Agreement in identifying Gram-negative bacteria at the genus level was 90.5%. Our findings corroborate that MALDI-TOF MS is an accurate, rapid and simple technique for identifying bovine mastitis pathogens. The availability of this methodology in some research institutions would represent a significant step toward increasing the diagnosis and epidemiological studies of bovine mastitis and other animal infectious diseases in Brazil.
机译：在该研究中，我们评估使用基质辅助激光解吸/离子释放时间质谱（MALDI-TOF MS）以鉴定巴西牛乳腺炎病例中分离的380个细菌。将MALDI-TOF MS确定与先前的生物化学测试和16S rRNA测序进行了识别。 MALDI-TOF MS达到了95.5％的可征。用于鉴定葡萄球菌的MALDI-TOF MS的准确性为93.2％。 MALDI-TOF MS与链球菌的生化鉴定之间的协议为96％，但这些技术与鉴定其他过氧化氢酶阴性革兰氏阳性COCC1的协议较低。在鉴定Gram阴性细菌在属级别的协议为90.5％。我们的研究结果证实了MALDI-TOF MS是一种准确，快速而简单地识别牛乳腺炎病原体。一些研究机构的这种方法的可用性将代表增加牛乳腺炎和巴西其他动物传染病的诊断和流行病学研究的重要一步。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号