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首页> 外文期刊>Journal of dairy research >N-acetyl -β-D-glucosaminidase activity in cow milk as an indicator of mastitis
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N-acetyl -β-D-glucosaminidase activity in cow milk as an indicator of mastitis

机译：牛奶中N-乙酰基-β-D-氨基葡萄糖苷酶活性指示乳腺炎

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摘要

Activity of lysosomal N-acetyl-β-D-glucosaminidase (NAGase) in milk has been used as an indicator of bovine mastitis. We studied NAGase activity of 808 milk samples from healthy quarters and quarters of cows with spontaneous subclinical and clinical mastitis. Associations between milk NAGase activity and milk somatic cell count (SCO, mastitis causing pathogen, quarter, parity, days in milk (DIM) and season were studied. In addition, the performance of NAGase activity in detecting clinical and subclinical mastitis and distinguishing infections caused by minor and major bacteria was investigated. Our results indicate that NAGase activity can be used to detect both subclinical and clinical mastitis with a high level of accuracy (0.85 and 0.99). Incomplete correlation between NAGase activity and SCC suggests that a substantial proportion of NAGase activity comes from damaged epithelial cells of the udder in addition to somatic cells. We therefore recommend determination of NAGase activity from quarter foremilk after at least six hours from the last milking using the method described. Samples should be frozen before analysis. NAGase activity should be interpreted according to DIM, at least during the first month of lactation. Based on the results of the present study, a reference value for normal milk NAGase activity of 0.1-1.04 pmoles 4-MU/min/μl for cows with ≥30 DIM (196 samples) could be proposed. We consider milk NAGase activity to be an accurate indicator of subclinical and clinical mastitis.
机译：牛奶中的溶酶体N-乙酰基-β-D-氨基葡萄糖苷酶（NAGase）的活性已被用作牛乳腺炎的指标。我们研究了健康的四分之一和四分之一自发性亚临床和临床乳腺炎的母牛的808份牛奶样品的NAGase活性。研究了乳NAGase活性与乳体细胞计数（SCO，引起乳腺炎的病原体，季，胎次，产奶天数（DIM）和季节之间的关系，此外，NAGase活性在检测临床和亚临床乳腺炎以及区分引起的感染方面的性能结果表明，NAGase活性可用于检测亚临床和临床乳腺炎，准确度很高（0.85和0.99）； NAGase活性与SCC之间不完全相关，表明NAGase的比例很高活性来自于乳房受损的上皮细胞和体细胞，因此，我们建议使用上述方法从最后一次挤奶至少六小时后，从四分之一前四分之一开始测定NAGase的活性，在分析之前应将样品冷冻。根据DIM的解释，至少在哺乳期的头一个月内。在本研究中，对于≥30 DIM的母牛（196个样本），正常乳NAGase活性的参考值为0.1-1.04 pmoles 4-MU / min /μl。我们认为牛奶NAGase活性是亚临床和临床乳腺炎的准确指标。

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  • 来源
    《Journal of dairy research》 |2016年第2期|219-227|共9页
  • 作者

    Mari Hovinen; Heli Simojoki; Reeta Poesoe; Jenni Suolaniemi; Piret Kalmus; Leena Suojala; Satu Pyoeraelae;

  • 作者单位

    Department of Production Animal Medicine, University of Helsinki, Helsinki, Finland;

    Department of Production Animal Medicine, University of Helsinki, Helsinki, Finland;

    Department of Veterinary Biosciences, University of Helsinki, Helsinki, Finland;

    Department of Production Animal Medicine, University of Helsinki, Helsinki, Finland;

    Department of Clinical Veterinary Medicine, Estonian University of Life Sciences, Estonia;

    Central Union of Agricultural Producers and Forest Owners MTK, Helsinki, Finland;

    Department of Production Animal Medicine, University of Helsinki, Helsinki, Finland;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    N-acetyl -β-D-glucosaminidase; cow milk; indicator of mastitis;

    机译：N-乙酰基-β-D-氨基葡萄糖苷酶;牛奶乳腺炎的指标;

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