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首页> 外文期刊>Journal of dairy science >Hepatic mRNA expression for genes related to somatotropic axis, glucose and lipid metabolisms, and inflammatory response of periparturient dairy cows treated with recombinant bovine somatotropin
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Hepatic mRNA expression for genes related to somatotropic axis, glucose and lipid metabolisms, and inflammatory response of periparturient dairy cows treated with recombinant bovine somatotropin

机译：重组牛生长激素处理与围产期奶牛生长激素轴，葡萄糖和脂质代谢以及炎症反应有关的基因的肝mRNA表达

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摘要

Objectives of this experiment were to evaluate the effects of recombinant bovine somatotropin (rbST) treatment of periparturient dairy cows on hepatic mRNA expression for genes related to the somatotropic axis, insulin, glucose, and lipid metabolism, inflammation, and oxidative stress. Holstein cows were enrolled in the experiment at 253 ± 3 d of gestation and assigned to 1 of 3 treatments: untreated control (n - 53), 87.5 mg of rbST (n = 56; rbST87.5), and 125 mg of rbST (n = 57; rbST125). Cows in the rbST87.5 and rbST125 treatments received weekly injections of rbST from -21 to 28 d relative to calving. A subsample of cows (control = 20, rbST87.5 = 20, rbST125 = 20) was randomly selected for collection of liver samples according to expected calving date, BCS, and previous lactation 305-d mature equivalent milk yield. Only cows that had liver sampled at -21 ± 3, -7 ± 3, and 7 ± 3 d relative to calving were used in the current experiment. Blood, sampled weekly from -28 to 21 d relative to calving, was used to determine the concentrations of growth hormone, insulin-like growth factor 1, insulin, Cortisol, fatty acids, β-hydroxybutyrate, glucose, haptoglobin, and tumor necrosis factor-α. Liver samples were used to determine hepatic mRNA expression of 50 genes. Treatment with rbST increased growth hormone concentrations during the postpartum period (control = 9.0 ± 0.7, rbST87.5 = 15.3 ± 1.0, rbST125 = 18.5 ± 1.3 ng/mL) and increased insulin-like growth factor 1 concentrations during the prepartum period (control = 107.4 ± 7.2, rbST87.5 = 126.9 ± 6.6, rbST125 = 139.4 ± 6.9 ng/mL). Control cows had greater postpartum concentrations of (3-hydroxybutyrate (control = 776.4 ± 64.0, rbST87.5 = 628.4 ± 59.7, rbST125 = 595.4 ± 60.9 μmol/L) than rbST cows. The rbST87.5 and rbST125 treatments upregulated the hepatic mRNA expression for somatotropic axis genes (GHR, GHR1A, IGF1, IGFBP3, and SOCS2) on d -7 relative to calving and upregulated the mRNA expression for SOCS2 on d 7. On d -7, rbST87.5 and rbST125 treatments increased mRNA expression for genes involved in hepatic lipid transport (ANGPTL4, APOA5, APOB100, and SCARB1) and downregulated mRNA expression for PPARD, which is involved in lipid storage. On d 7, rbST tended to upregulate the mRNA expression for genes involved in gluconeogenesis (PCK1) and fatty acid β-oxidation (ACOX1), and downregulated the mRNA expression for genes involved in inflammation (TNFRSF1A, ICAM1, CXCL1, MYD88, HIF1A, IL1RN, NFKBIA, and SOCS3) and oxidative stress (XBP1). Administration of rbST during the periparturient period may improve liver function and health by increasing hepatic capacity for gluconeogenesis and lipid transport and by reducing inflammation and oxidative stress.
机译：该实验的目的是评估重组牛生长激素（rbST）处理围产期奶牛对与生长激素轴，胰岛素，葡萄糖和脂质代谢，炎症和氧化应激有关的基因的肝mRNA表达的影响。荷斯坦奶牛在妊娠253±3 d时参加了实验，并分配为以下3种处理方法之一：未处理的对照组（n-53），87.5 mg rbST（n = 56; rbST87.5）和125 mg rbST（ n = 57； rbST125）。相对于产犊，采用rbST87.5和rbST125处理的母牛每周接受rbST的注射时间为-21至28天。根据预期产犊日期，BCS和先前泌乳期305 d成熟同等牛奶产量，随机选择母牛的一个子样本（对照= 20，rbST87.5 = 20，rbST125 = 20）来收集肝脏样品。在本实验中，仅使用相对于产犊在-21±3，-7±3和7±3 d采样肝脏的母牛。相对于产犊，每周从-28到21天抽取血液，用于确定生长激素，胰岛素样生长因子1，胰岛素，皮质醇，脂肪酸，β-羟基丁酸酯，葡萄糖，触珠蛋白和肿瘤坏死因子的浓度-α。肝样品用于确定50个基因的肝mRNA表达。 rbST治疗在产后期间增加生长激素浓度（对照组= 9.0±0.7，rbST87.5 = 15.3±1.0，rbST125 = 18.5±1.3 ng / mL），在产前期间增加胰岛素样生长因子1浓度（对照组） = 107.4±7.2，rbST87.5 = 126.9±6.6，rbST125 = 139.4±6.9 ng / mL）。对照奶牛的产后浓度（3-羟基丁酸酯（对照= 776.4±64.0，rbST87.5 = 628.4±59.7，rbST125 = 595.4±60.9μmol/ L））比rbST奶牛更高。rbST87.5和rbST125处理上调了肝mRNA相对于产犊，d -7上促生长轴基因（GHR，GHR1A，IGF1，IGFBP3和SOCS2）的表达，并在d 7上调SOCS2的mRNA表达。在d -7上，rbST87.5和rbST125处理增加了参与肝脏脂质转运的基因（ANGPTL4，APOA5，APOB100和SCARB1）和PPARD的mRNA表达下调（与脂质存储有关），第7天，rbST倾向于上调糖原异生性基因（PCK1）和mRNA的表达。脂肪酸β-氧化（ACOX1），并下调与炎症有关的基因（TNFRSF1A，ICAM1，CXCL1，MYD88，HIF1A，IL1RN，NFKBIA和SOCS3）和氧化应激（XBP1）的mRNA表达。期间可能会改善生活通过增加肝糖异生和脂质转运的能力以及减少炎症和氧化应激来实现功能和健康。

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  • 来源
    《Journal of dairy science》 |2017年第5期|3983-3999|共17页
  • 作者

    P. R. B. Silva; W. J. Weber; B. A. Crooker; R. J. Collier; W. W. Thatcher; R. C. Chebel;

  • 作者单位

    Department of Animal Science, University of Minnesota, Saint Paul 55108;

    Department of Animal Science, University of Minnesota, Saint Paul 55108;

    Department of Animal Science, University of Minnesota, Saint Paul 55108;

    School of Animal and Comparative Biomedical Sciences, The University of Arizona, Tucson 85721;

    Department of Animal Sciences, University of Florida, Gainesville 32608;

    Department of Animal Science, University of Minnesota, Saint Paul 55108,Department of Animal Sciences, University of Florida, Gainesville 32608,Department of Large Animal Clinical Sciences, University of Florida, Gainesville 32608;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    periparturient cows; recombinant bovine somatotropin; hepatic gene expression;

    机译：围产期母牛重组牛生长激素肝基因表达;

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