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首页> 外文期刊>Journal of dairy science >Rumen-protected methionine compared with rumen-protected choline improves immunometabolic status in dairy cows during the peripartal period
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Rumen-protected methionine compared with rumen-protected choline improves immunometabolic status in dairy cows during the peripartal period

机译：与瘤胃保护的胆碱相比，瘤胃保护的蛋氨酸可改善围产期奶牛的免疫代谢状态

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摘要

The immunometabolic status of peripartal cows is altered due to changes in liver function, inflammation, and oxidative stress. Nutritional management during this physiological state can affect the biological components of immunometabolism. The objectives of this study were to measure concentrations of biomarkers in plasma, liver tissue, and milk, and also polymorphonu-clear leukocyte function to assess the immunometabolic status of cows supplemented with rumen-protected methionine (Met) or choline (CHOL). Forty-eight multiparous Holstein cows were used in a randomized complete block design with 2×2 factorial arrangement of Met (Smartamine M, Adisseo NA, Alpharetta, GA) and CHOL (ReaShure, Balchem Inc., New Hampton, NY) level (with or without). Treatments (12 cows each) were control (CON), no Met or CHOL; CON and Met (SMA); CON and CHOL (REA); and CON and Met and CHOL (MIX). Prom -50 to -21 d before expected calving, all cows received the same diet [1.40 Meal of net energy for lactation (NE_L)/kg of DM] with no Met or CHOL. From -21 d to calving, cows received the same close-up diet (1.52 Meal of NE_L/kg of DM) and were assigned randomly to each treatment. From calving to 30 d, cows were on the same postpartal diet (1.71 Meal of NE_L/kg of DM) and continued to receive the same treatments until 30 d. The Met supplementation was adjusted daily at 0.08% DM of diet, and CHOL was supplemented at 60 g/cow per day. Liver (-10, 7, 21, and 30 d) and blood (-10, 4, 8, 20, and 30 d) samples were harvested for biomarker analyses. Neutrophil and monocyte phagocytosis and oxidative burst were assessed at d 1, 4, 14, and 28 d. The Met-supplemented cows tended to have greater plasma paraoxonase. Greater plasma albumin and IL-6 as well as a tendency for lower haptoglobin were detected in Met- but not CHOL-supplemented cows. Similarly, cows fed Met compared with CHOL had greater concentrations of total and reduced glutathione (a potent intracellular antioxidant) in liver tissue. Upon a pathogen challenge in vitro, blood polymorphonuclear leukocyte phagocytosis capacity and oxidative burst activity were greater in Met-supplemented cows. Overall, liver and blood biomarker analyses revealed favorable changes in liver function, inflammation status, and immune response in Met-supplemented cows.
机译：由于肝功能，炎症和氧化应激的改变，围产期母牛的免疫代谢状态发生了改变。在这种生理状态下的营养管理会影响免疫代谢的生物学成分。这项研究的目的是测量血浆，肝组织和牛奶中生物标志物的浓度，以及多形觉通明的白细胞功能，以评估补充有瘤胃保护蛋氨酸（Met）或胆碱（CHOL）的母牛的免疫代谢状态。将48头荷斯坦奶牛用于Met（Smartamine M，Adisseo NA，Alpharetta，GA）和CHOL（ReaShure，Balchem Inc.，New Hampton，NY）水平的2×2因数排列的随机完整区组设计（或没有）。治疗（每头12头母牛）为对照（CON），无Met或CHOL； CON和Met（SMA）； CON和CHOL（REA）；以及CON和Met and CHOL（MIX）。在预期产犊前的-50至-21 d舞会上，所有母牛都接受相同的日粮[1.40哺乳净能量餐（NE_L）/ kg DM]，无Met或CHOL。从-21天到产犊，母牛接受相同的特写饮食（1.52餐NE_L / kg DM），并随机分配给每种处理。从产犊到第30天，母牛采用相同的产后饮食（1.71顿NE_L / kg DM膳食），并继续接受相同的治疗直至30 d。每天调整Met补充剂量为饮食的0.08％DM，每天补充CHOL的剂量为60 g /牛。收集肝脏（-10、7、21和30 d）和血液（-10、4、8、20和30 d）样本进行生物标志物分析。在第1、4、14和28天评估中性粒细胞和单核细胞的吞噬作用和氧化爆发。补充了Met的母牛倾向于具有更高的血浆对氧磷酶。在补充了Met但未添加CHOL的母牛中检测到更高的血浆白蛋白和IL-6以及更低的触珠蛋白趋势。同样，与CHOL相比，饲喂Met的母牛肝脏组织中的总谷胱甘肽浓度更高且谷胱甘肽含量降低（一种有效的细胞内抗氧化剂）。在体外进行病原体攻击后，补充Met的牛的血液多形核白细胞吞噬能力和氧化爆发活性更高。总体而言，肝脏和血液生物标志物分析显示，补充了Met的奶牛的肝功能，炎症状态和免疫反应发生了有利的变化。

著录项

  • 来源
    《Journal of dairy science》 |2016年第11期|8956-8969|共14页
  • 作者

    Z. Zhou; O. Bulgari; M. Vailati-Riboni; E. Trevisi; M. A. Ballou; F. C. Cardoso; D. N. Luchini; J. J. Loor;

  • 作者单位

    Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana 61801;

    Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana 61801,Dipartimento di Medicina Molecolare e Traslazionale, Universita degli Studi di Brescia, 25121 Brescia, Italy;

    Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana 61801;

    lstituto di Zootecnica Facolta di Scienze Agrarie, Alimentari e Ambientali, Universita Cattolica del Sacra Cuore, 29122, Piacenza, Italy;

    Department of Animal Sciences, Texas Tech University, Lubbock 79409;

    Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana 61801;

    Adisseo, Alpharetta, GA 30022;

    Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana 61801;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    inflammation; oxidative stress; transition cow; nutrition;

    机译：炎;氧化应激过渡牛营养;

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